Protein Info for GFF382 in Sphingobium sp. HT1-2

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 564 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 117 to 286 (170 residues), 114.5 bits, see alignment E=2.1e-37 PF00990: GGDEF" amino acids 120 to 282 (163 residues), 123.9 bits, see alignment E=5.4e-40 PF00563: EAL" amino acids 303 to 540 (238 residues), 233.7 bits, see alignment E=2e-73

Best Hits

KEGG orthology group: None (inferred from 82% identity to sch:Sphch_0010)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (564 amino acids)

>GFF382 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Sphingobium sp. HT1-2)
MQGVTVKSRAAAFACAAGALVFILSLLLGHGAADDLVDVGRSLIIAILCGTMSWASAQRS
IAATASAIDTATERLLAAAHGDLHSHVPAEIGLELPDLSVAMESLFSQVRTNLDHVQTLA
LFDQVTGLANRTSFCRQVERLLVERGGAGPAALFFIDLDGFKAVNDTLGHAAGDQLLARV
AGRLREVVMAQVSAGLGDAVIGRLAGDEFTMFFPALPSREAATRIARAIQFALGERFELG
SQHVDLGASIGIACYPDHGDSLSALLRSADIAMYHAKHEGRGRAEMFTAELALEAADRAE
LERDLLLGLERDEFLLEFQPQIEMSHGGVAQAVTAEALVRWVHPERALIMPGAFVPVAEE
SGMIVALGDWVMSRVCETAARWARSGVHQRIAINISTRELAQADFFLRLRHAMATHQTPP
PMLELEITESLAMDMDDRVLGQLSALRQDGVRIAIDDFGTGYSNLARLRELPVDRVKIDR
SLVRDIATSAEARTICSAVVGLIQGLGMEVVIEGVETQAQMDMLRVIGCTLFQGYHLARP
MGEAQYLDRYAAAPTVEPTERRSL