Protein Info for PS417_19535 in Pseudomonas simiae WCS417

Annotation: dihydroneopterin aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 253 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF10411: DsbC_N" amino acids 30 to 86 (57 residues), 30.2 bits, see alignment E=4.2e-11 PF13462: Thioredoxin_4" amino acids 115 to 182 (68 residues), 25.5 bits, see alignment E=2.1e-09 PF13098: Thioredoxin_2" amino acids 121 to 250 (130 residues), 44.8 bits, see alignment E=2e-15

Best Hits

Swiss-Prot: 65% identical to DSBG_PSEAE: Thiol:disulfide interchange protein DsbG (dsbG) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03805, thiol:disulfide interchange protein DsbG (inferred from 95% identity to pfs:PFLU4384)

MetaCyc: 45% identical to protein sulfenic acid reductase and chaperone DsbG (Escherichia coli K-12 substr. MG1655)
Protein disulfide-isomerase. [EC: 5.3.4.1]; RXN-20019 [EC: 5.3.4.1]

Predicted SEED Role

"Thiol:disulfide interchange protein DsbG precursor" in subsystem Periplasmic disulfide interchange

Isozymes

Compare fitness of predicted isozymes for: 5.3.4.1

Use Curated BLAST to search for 5.3.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UPA2 at UniProt or InterPro

Protein Sequence (253 amino acids)

>PS417_19535 dihydroneopterin aldolase (Pseudomonas simiae WCS417)
MPRLCHLLTLLPLTLAATLAQAADWPAPIKQIEAKGAKILGKFDAPSGLTGYAAQYQNRG
MALYLTADGKSVIAGSLYDAQGNDLSSAPLEKLVYAPMAKEVWAKMEKSSWIQDGDVKAP
RIVYLFSDPNCPYCNMFWEQARPWIKAGKVQLRHIMVGIIREDSPGKSAALFAAKDPQKA
LEEHEAAGKGSKLQALEKIPADIEAKLDGNMKLMDGLELSATPAIFYLDDKGGLQQQQGA
PSPEKLGKILGPK