Protein Info for PS417_19525 in Pseudomonas simiae WCS417

Annotation: thiol:disulfide interchange protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 575 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 161 to 190 (30 residues), see Phobius details amino acids 202 to 225 (24 residues), see Phobius details amino acids 237 to 259 (23 residues), see Phobius details amino acids 280 to 310 (31 residues), see Phobius details amino acids 317 to 341 (25 residues), see Phobius details amino acids 353 to 373 (21 residues), see Phobius details amino acids 379 to 399 (21 residues), see Phobius details amino acids 408 to 429 (22 residues), see Phobius details PF11412: DsbD_N" amino acids 28 to 132 (105 residues), 90.4 bits, see alignment E=2.6e-29 PF02683: DsbD_TM" amino acids 161 to 375 (215 residues), 268.4 bits, see alignment E=1.2e-83 PF13386: DsbD_2" amino acids 166 to 358 (193 residues), 30.6 bits, see alignment E=9.4e-11 PF13899: Thioredoxin_7" amino acids 467 to 540 (74 residues), 48.4 bits, see alignment E=2.4e-16 PF13098: Thioredoxin_2" amino acids 472 to 566 (95 residues), 32.2 bits, see alignment E=3.5e-11 PF00085: Thioredoxin" amino acids 475 to 563 (89 residues), 30.9 bits, see alignment E=6.7e-11

Best Hits

Swiss-Prot: 74% identical to DSBD_PSEF5: Thiol:disulfide interchange protein DsbD (dsbD) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K04084, thiol:disulfide interchange protein DsbD [EC: 1.8.1.8] (inferred from 93% identity to pfs:PFLU4382)

Predicted SEED Role

"Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8)" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange (EC 1.8.1.8)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.8.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7U4A6 at UniProt or InterPro

Protein Sequence (575 amino acids)

>PS417_19525 thiol:disulfide interchange protein (Pseudomonas simiae WCS417)
MRHLFTFLLVLFAGFAQAAPGNPFETKPDFLPVGKAFTFTSERLESGETQLYWQIADGYY
LYKQRMKFDGLAKQPTLPQGEAHSDEFFGDQEVYRQGLEVKIPAGATGQVKLGWQGCADA
GLCYPPQSITVDLGGNPAVAATAEAQDQSLASGLQQRSLGWSLLIFFGLGLLLAFAPCSL
PMLPILAGLVVGSGASPRRGFALASSYVVCMALVYAALGVLAALLGGNLAAWLQTPWILG
SFAALFVLLALPMFGFFELQLPAFLRDRLDNVSRQQSGGSLVGAGILGALSGLLVGPCMT
APLAGALLYIAQSGNALHGGLILFAMGIGIGIPLLLLVTVGNRFLPKPGTWMNVLKGIFG
FLFLGTAVLMIRPVVDESLWIGLWGTLALVMAYCGWTLAREYGLAAKVFGAGSLVLGLWG
TLLVVGAAGGSDDLWQPLKVYSASRTAAAPSAHDAFMTINDPVVLQSQLDSAKAQGQWVL
VDYYADWCVSCKIMEKQVFGKPEVMDALKDVRLLRLDVTADNAASRELLGRYKVPGPPSF
VWIGPDGEERRAQRITGEVDAAAFLQRWTQTRDAL