Protein Info for GFF3813 in Sphingobium sp. HT1-2

Annotation: TonB-dependent receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 898 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF07715: Plug" amino acids 53 to 175 (123 residues), 77.2 bits, see alignment E=1.3e-25 PF00593: TonB_dep_Rec_b-barrel" amino acids 342 to 846 (505 residues), 128.4 bits, see alignment E=7.2e-41

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 84% identity to sjp:SJA_C1-10610)

Predicted SEED Role

"TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (898 amino acids)

>GFF3813 TonB-dependent receptor (Sphingobium sp. HT1-2)
MKYQGLFSCSAIAIALSYSMPVLAQDVAPQAAAAETPGQTIIVTGTRRTDRTVAESPTPI
DVYSGEELQKQGVADMNQVIQNLVPSFSVARYAIGDGSSFVRPPNLRGLAPDQTLVMVNG
KRMHRSALVQIGANAQSAGAHSADLAQIPAIAVRSVEVLRDGASAQYGSDAIAGVINMAL
RDDTDGISGYARYGQYYRGDGEDYQVALNGGFKLGDNGFINISGEYVNAGKTSRGVTRPG
ALELSQEQPDIGVPKLAQRYGNPKMESYRFFVNGGFDLGDDSSIYFFGNYGHSFQEESFN
YRQSVNSANFGLNGIFNDYFTDFDNTTPGIQTGAAGGNVYAPNDYDPTTRDYTQVSDCSD
PLVQNWSCVYSGGFTPIFFGKIDDISGTVGYKGTLGFGLNYDLSGSYGQSTLKYTMNGTM
NPSLGITSPTEFYLGKLEQRETLFNADFSYPWEVGLASPITIAFGGQYFRESYELGLGDA
ASYAIGPYTGNLVFHQDGTPVLNADGSQLSVALPVGANGFPGYGPAIAGESSRNSKGLYI
DVEGDITEAFSLGFAGRYEHLSDFGNSTTGKVSARYAIVPDVFAVRGTLSTGFRAPTPGQ
TNTSSIATTFNPGNPNAIEFMTLPVASPVAQYLGAVPLKPEESVSFSAGTVFTPAPGASL
TIDYYNIKVKDRIGTTGSRVITDDQRPELQALGLANYATVNEVQFLTNAFDTRTQGVDVV
GSYRFSTDSMGTFNTTLAFNYNKTKVIRVGLDPDTGSPIISDTRIMQLQNTLPKTRVILT
ENWTSGALSVLGRANWYGKYKVYDDGTGANGESYVNQSFGSEFVFDLEVSYQLNDNFTLS
AGAQNLFSNYPDKYDNRAGGLGPVYASTGGRFTGDIYPDVSPFGFNGGFWYAKVGFKF