Protein Info for GFF3811 in Sphingobium sp. HT1-2

Annotation: tRNA (guanine(37)-N(1))-methyltransferase (EC 2.1.1.228)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 245 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR00088: tRNA (guanine(37)-N(1))-methyltransferase" amino acids 6 to 233 (228 residues), 248.1 bits, see alignment E=3.5e-78 PF01746: tRNA_m1G_MT" amino acids 25 to 222 (198 residues), 154.2 bits, see alignment E=1.8e-49

Best Hits

Swiss-Prot: 80% identical to TRMD_SPHWW: tRNA (guanine-N(1)-)-methyltransferase (trmD) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K00554, tRNA (guanine-N1-)-methyltransferase [EC: 2.1.1.31] (inferred from 88% identity to sch:Sphch_1635)

MetaCyc: 43% identical to tRNA m1G37 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-12458 [EC: 2.1.1.228]

Predicted SEED Role

"tRNA (Guanine37-N1) -methyltransferase (EC 2.1.1.31)" in subsystem Ribosome biogenesis bacterial or Wyeosine-MimG Biosynthesis (EC 2.1.1.31)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.228 or 2.1.1.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (245 amino acids)

>GFF3811 tRNA (guanine(37)-N(1))-methyltransferase (EC 2.1.1.228) (Sphingobium sp. HT1-2)
MSFAAQILTLYPEMFPGPLGVSLAGRALAEGKWSCDPIQMRGFATDKHRTVDDTPAGGGA
GMVLKPDILARGIDHALAQRPDLPILAMTPRGKPITQARIRALADGPGVTLLCGRFEGFD
ERIFDARPIEQISMGDIILSGGEMGALMLLDACIRLLPGVMGAASSGVEESFETGLLEYP
HYTRPVEWEGRRIPEVLRSGDHAKIAAWRKQQAEADTRLRRPDLWERHIGVRDQSPSGAQ
RENKD