Protein Info for GFF3810 in Variovorax sp. SCN45

Annotation: Conserved membrane protein in copper uptake, YcnI / Copper metallochaperone PCu(A)C, inserts Cu(I) into cytochrome oxidase subunit II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 303 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF07987: DUF1775" amino acids 28 to 160 (133 residues), 81.3 bits, see alignment E=9.4e-27 PF04314: PCuAC" amino acids 171 to 276 (106 residues), 125.7 bits, see alignment E=7.5e-41

Best Hits

KEGG orthology group: K09796, hypothetical protein (inferred from 90% identity to vpe:Varpa_5430)

Predicted SEED Role

"Copper metallochaperone, bacterial analog of Cox17 protein" in subsystem Biogenesis of cytochrome c oxidases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (303 amino acids)

>GFF3810 Conserved membrane protein in copper uptake, YcnI / Copper metallochaperone PCu(A)C, inserts Cu(I) into cytochrome oxidase subunit II (Variovorax sp. SCN45)
MTKPMAIATLRTLAACAMLAGTTAALAHVTLPPGGATVGSDYNAAFRVGHACEGAKATTG
LAVRLPKGFVLSDAQARKGWKLDVTKAGDGEVRWTAETPGNALPGSERSEFVLRGKVPAT
PGTLWFKVLQTCDTGVADWAEVPASGNSTAGLKSPAAKLVVVAQGVATVDVRDGWVRQSV
PGQSGTGAFMKLTAPTGTKLVSISTPAAGTAEVHEMKMEGDVMKMRELPGGLDLPAGQTV
ELKPGGYHVMMMDLKGALTKGATVPMTLKFEDAKGVKTALDVTLPVGAPEGADAAGSAHQ
HKH