Protein Info for GFF381 in Variovorax sp. SCN45

Annotation: Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosam ine O-acyltransferase (EC 2.3.1.129)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01852: acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase
  • amino acids 4 to 260 (257 residues), 314.1 bits, E=3e-98
• PF00132: Hexapep
  • amino acids 105 to 139 (35 residues), 30 bits, 2.9e-11
• PF13720: Acetyltransf_11
  • amino acids 176 to 260 (85 residues), 82.5 bits, E=2.4e-27

Best Hits

Swiss-Prot: 92% identical to LPXA_VARPS: Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) from Variovorax paradoxus (strain S110)

KEGG orthology group: K00677, UDP-N-acetylglucosamine acyltransferase [EC: 2.3.1.129] (inferred from 91% identity to vpe:Varpa_3315)

MetaCyc: 51% identical to acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (Escherichia coli K-12 substr. MG1655)
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase. [EC: 2.3.1.129]

Predicted SEED Role

"Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (EC 2.3.1.129)" in subsystem KDO2-Lipid A biosynthesis (EC 2.3.1.129)

MetaCyc Pathways

• lipid IV_A biosynthesis (P. gingivalis) (9/9 steps found)
• superpathway of (Kdo)₂-lipid A biosynthesis (14/17 steps found)
• lipid IV_A biosynthesis (E. coli) (6/6 steps found)
• lipid IV_A biosynthesis (H. pylori) (6/6 steps found)
• lipid IV_A biosynthesis (P. putida) (6/6 steps found)
• lipid IV_A biosynthesis (Vibrio cholerae serogroup O1 El Tor) (6/6 steps found)
• lipid IV_A biosynthesis (generic) (6/6 steps found)
• superpathway of Kdo₂-lipid A biosynthesis (14/25 steps found)

KEGG Metabolic Maps

• Lipopolysaccharide biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.129

Use Curated BLAST to search for 2.3.1.129

Protein Sequence (262 amino acids)

>GFF381 Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosam ine O-acyltransferase (EC 2.3.1.129) (Variovorax sp. SCN45)
MTQVHSTAIVDPQAELDASVSVGPYTVIGPHVRIAAGTTVGAHCVIEGRTTIGRDNRIFQ
FSSLGAVPQDKKYAGEPTELVIGDRNVIREFCTFNLGVPGAGGVTRVGSDNWIMAYTHIA
HDCRVDNHTTLSNNTTLAGHVHLADWVTIGGLTGIHQFVSVGAHAMVGFASAISQDVPPF
MLVDGNPLAVRGFNIVGLRRRDFSAQRLAAVKQMHRLLYRQGKTLEEARTDIAALATEMP
EAAGDVALMESFLANSTRGIAR