Protein Info for GFF3801 in Sphingobium sp. HT1-2

Annotation: Multidrug efflux system EmrAB-OMF, inner-membrane proton/drug antiporter EmrB (MFS type)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 508 transmembrane" amino acids 17 to 39 (23 residues), see Phobius details amino acids 56 to 77 (22 residues), see Phobius details amino acids 84 to 108 (25 residues), see Phobius details amino acids 113 to 132 (20 residues), see Phobius details amino acids 143 to 164 (22 residues), see Phobius details amino acids 170 to 192 (23 residues), see Phobius details amino acids 204 to 223 (20 residues), see Phobius details amino acids 235 to 256 (22 residues), see Phobius details amino acids 275 to 297 (23 residues), see Phobius details amino acids 306 to 327 (22 residues), see Phobius details amino acids 338 to 356 (19 residues), see Phobius details amino acids 371 to 393 (23 residues), see Phobius details amino acids 405 to 424 (20 residues), see Phobius details amino acids 475 to 495 (21 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 20 to 493 (474 residues), 423.3 bits, see alignment E=6.4e-131 PF07690: MFS_1" amino acids 22 to 415 (394 residues), 182.6 bits, see alignment E=5.1e-58

Best Hits

Swiss-Prot: 44% identical to EMRB_ECO57: Multidrug export protein EmrB (emrB) from Escherichia coli O157:H7

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 73% identity to sch:Sphch_1724)

MetaCyc: 44% identical to multidrug efflux pump membrane subunit EmrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (508 amino acids)

>GFF3801 Multidrug efflux system EmrAB-OMF, inner-membrane proton/drug antiporter EmrB (MFS type) (Sphingobium sp. HT1-2)
VSGPETFNLLSPRTRTLAGLVLALSNFMVVLDLTIANVSVPHIAGNLGISGDQGTWIITS
YAVAEAICVPLTGWLALRFGVVRTFIWAMIGFGFFSMMCGLSTTLGMIVAARLGQGICGG
PIMPMSQTLLLRIFPPEMRPRAMGLWAMTTLLGPAMGPIVGGYISDNWSWHWIFFINLPI
AALCVFAAMAMLRPVETDTAHQPIDKIGLCLLIFWIGCLQVMLDIGRDHDWFSDPLILTL
AILAGIGFIVFIIWELTEEHPIVDLRVFRHIGFSSGVFTLALAFGAYFSSIVVIPQWLQM
SMGYTATWSGIVTAFTAMAAVMSAPFAARFTGKVDPRIMISGAVFWLGCMTLWRAHWTSG
IDFFGLALPQFVQGFAMPFFMIPLTTLTLGSVPPSETASAAGLQNFLRTMAIAIATSLVL
TGWSDSQRVSRNELAGALQPDDAQRALSGMGMTTEQIRQTISNLVEQEAIAISVDYVFFI
SALVLFAAAVIVWLAPKPSGPVDTSAAH