Protein Info for GFF3796 in Sphingobium sp. HT1-2

Annotation: Precorrin-2 oxidase (EC 1.3.1.76) @ Sirohydrochlorin ferrochelatase activity of CysG (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K02302, uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 2.1.1.107 4.99.1.4] (inferred from 71% identity to sjp:SJA_C1-28660)

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis or Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase (EC 1.3.1.76, EC 2.1.1.107, EC 4.99.1.4)

MetaCyc Pathways

adenosylcobalamin biosynthesis II (aerobic) (28/33 steps found)
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (13/13 steps found)
siroheme biosynthesis (4/4 steps found)
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (11/15 steps found)
adenosylcobalamin biosynthesis I (anaerobic) (25/36 steps found)
factor 430 biosynthesis (3/7 steps found)

KEGG Metabolic Maps

Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.76, 2.1.1.107, 4.99.1.4

Use Curated BLAST to search for 1.3.1.76 or 2.1.1.107 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (252 amino acids)

>GFF3796 Precorrin-2 oxidase (EC 1.3.1.76) @ Sirohydrochlorin ferrochelatase activity of CysG (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) (Sphingobium sp. HT1-2)
MHSLPVFLRLEGRAVILTGEGEAADAKRRLLERAGARIVGEDDADARVAIVSDGDAAVVA
RLRARGVLVNATDKPDLCDFTLPAIVDRNPVLIAIGTGGASAGLAAALRQRIEALLPSGL
GDLAQALFAARGRLRDLWPDAGARRQAIGKALAPGGAIDPLGFDPDVDVWLAENPEADNS
ELYLVRLTSADPDDLSVRDARMLALADRVYHDASVAPAILDRARADAERIAADGPPERLE
TGLSLWVSSAAR