Protein Info for GFF3793 in Sphingobium sp. HT1-2

Annotation: Argininosuccinate lyase (EC 4.3.2.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00838: argininosuccinate lyase
  • amino acids 1 to 453 (453 residues), 590.6 bits, E=1.2e-181
• PF00206: Lyase_1
  • amino acids 11 to 298 (288 residues), 264.4 bits, E=1.6e-82
• PF14698: ASL_C2
  • amino acids 361 to 430 (70 residues), 93.1 bits, E=1.2e-30

Best Hits

Swiss-Prot: 80% identical to ARLY_SPHWW: Argininosuccinate lyase (argH) from Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273)

KEGG orthology group: K01755, argininosuccinate lyase [EC: 4.3.2.1] (inferred from 95% identity to sjp:SJA_C1-28690)

MetaCyc: 49% identical to argininosuccinate lyase (Escherichia coli K-12 substr. MG1655)
Argininosuccinate lyase. [EC: 4.3.2.1]

Predicted SEED Role

"Argininosuccinate lyase (EC 4.3.2.1)" in subsystem Arginine Biosynthesis extended (EC 4.3.2.1)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
• nitric oxide biosynthesis II (mammals) (2/3 steps found)
• superpathway of arginine and polyamine biosynthesis (12/17 steps found)
• urea cycle (3/5 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• canavanine biosynthesis (2/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.2.1

Protein Sequence (455 amino acids)

>GFF3793 Argininosuccinate lyase (EC 4.3.2.1) (Sphingobium sp. HT1-2)
MWGGRFAAGPASIMREINASIPFDKRLWKQDIAGSKAHVAMLAKQGIVDGEDAQAITEGL
NRIAAEYEANGVPVNLDLEDIHMVTESRLAELIGPAAGRLHTARSRNDQVATDFRLWVRD
AMDEVMAGLAGLQQALLARAEEHVDAVMPGFTHLQSAQPVTLGHHLMAYYEMVRRDRSRF
ADARVRLDECPLGAAALAGTGFPIDRHATAAALGFAKPTDNSLDSVSDRDFALDYLMAAT
QASLHLSRLAEEFIIWASQPFGFVKLPDAYSTGSSIMPQKRNPDAAELVRGHAGRIMGCM
NALCVTMKGLPLAYSKDMQDDKPPVFEAHDLLGLSIAAMTGMVETVTFRTDRMRGLAESG
FATATDLADWLVREGDIPFREAHHITGRAVAAAEEAGVQLADLPLDRLKAIDARIDDRIY
AVLTVDASVASRKSHGGTAPDQVRARIAAARATQE