Protein Info for GFF3790 in Xanthobacter sp. DMC5
Annotation: 3-hydroxy-3-isohexenylglutaryl-CoA/hydroxy-methyl glutaryl-CoA lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 62% identical to HMGCL_BOVIN: Hydroxymethylglutaryl-CoA lyase, mitochondrial (HMGCL) from Bos taurus
KEGG orthology group: K01640, hydroxymethylglutaryl-CoA lyase [EC: 4.1.3.4] (inferred from 84% identity to xau:Xaut_1854)MetaCyc: 61% identical to Hydroxymethylglutaryl-CoA lyase, mitochondrial (Homo sapiens)
Hydroxymethylglutaryl-CoA lyase. [EC: 4.1.3.4]
Predicted SEED Role
"Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.1.3.4)
MetaCyc Pathways
- L-leucine degradation I (5/6 steps found)
- mevalonate degradation (2/2 steps found)
- ketogenesis (4/5 steps found)
KEGG Metabolic Maps
- Butanoate metabolism
- Synthesis and degradation of ketone bodies
- Valine, leucine and isoleucine degradation
Isozymes
No predicted isozymesUse Curated BLAST to search for 4.1.3.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (301 amino acids)
>GFF3790 3-hydroxy-3-isohexenylglutaryl-CoA/hydroxy-methyl glutaryl-CoA lyase (Xanthobacter sp. DMC5) MARPASVRIVEVGPRDGLQNEPGIVPLDAKVALIDALADAGLKMIEAGSFVSPKWVPQMA DTAEVLGAINRRAGVRYTVLAPNLKGLELALACGIEEVAVFTAASEAFTSKNINCTIAES LERFTPLIDLARARGVAVRGYVSCALGCPYQGEVPVAKVVEVSEALTAMGCYEVSVSDTI GVGTPDKAQAVTGAVAERIGLERTAIHFHDTYGQALANVLACLDIGVASVDSAVAGLGGC PYAKGASGNLATEDLVYMLNGLGIETGVDLDRLAAAGRAITAALGRTPASKVAQALAAKC A