Protein Info for PS417_01930 in Pseudomonas simiae WCS417

Annotation: arginyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 578 PF03485: Arg_tRNA_synt_N" amino acids 4 to 90 (87 residues), 87.4 bits, see alignment E=1.2e-28 TIGR00456: arginine--tRNA ligase" amino acids 6 to 578 (573 residues), 558.2 bits, see alignment E=9.4e-172 PF00750: tRNA-synt_1d" amino acids 102 to 448 (347 residues), 422.8 bits, see alignment E=1.5e-130 PF05746: DALR_1" amino acids 462 to 578 (117 residues), 115.6 bits, see alignment E=2.1e-37

Best Hits

Swiss-Prot: 94% identical to SYR_PSEFS: Arginine--tRNA ligase (argS) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K01887, arginyl-tRNA synthetase [EC: 6.1.1.19] (inferred from 94% identity to pfs:PFLU0401)

Predicted SEED Role

"Arginyl-tRNA synthetase (EC 6.1.1.19)" (EC 6.1.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TYT6 at UniProt or InterPro

Protein Sequence (578 amino acids)

>PS417_01930 arginyl-tRNA synthetase (Pseudomonas simiae WCS417)
MKDTIRQLIQQALTQLVNEGVLPEGLTPAIQVENTRDKTHGDFASNIAMMLSKPAGMKPR
DLAEKIIAALPVDESVSKAEIAGPGFINFFQNTQALASRLDAALADAKIGVHKAGPLQRV
VIDLSAPNLAKEMHVGHLRSTIIGDGVARVLEYLGDTVIRQNHVGDWGTQFGMLMAYLQE
NPITSNELSDLENFYRAAKKRFDESEEFAERARGLVVKLQAGDKECLALWNRFREISLSH
CQEIYELLNVKLTMADVMGESAYNDDLINVVNDLKAKGLLVESNGAQCVFLEEFKTADGE
PLPVIIVKADGGYLYATTDLAAVRYRSGVLKADRALYFVDQRQALHFQQVFEVARRAGFV
THPMHMEHMGFGTMNGADGRPFKTRDGGTVKLIDLLTEAQERAYNLVKEKNPELAEADLR
NIARVVGIGAVKYADLSKHRTSDYSFNFELMLNFEGNTAPYLLYAYTRVAGVFRKLGKDF
SEVEGQINLEATHEQELAAKLAQFGEVLNSVGEKGTPHILCTYLYEVAGLFSSFYENCPI
LTADDEAQKQSRLRLAALAGRTLKQGLELLGLETLERM