Protein Info for GFF3784 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: S-formylglutathione hydrolase (EC 3.1.2.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02821: S-formylglutathione hydrolase
  • amino acids 2 to 276 (275 residues), 391.7 bits, E=1.1e-121
• PF00756: Esterase
  • amino acids 22 to 271 (250 residues), 249.2 bits, E=8.2e-78
• PF00326: Peptidase_S9
  • amino acids 127 to 276 (150 residues), 29.7 bits, E=6.9e-11

Best Hits

Swiss-Prot: 89% identical to SFGH2_ECOUT: S-formylglutathione hydrolase YeiG (yeiG) from Escherichia coli (strain UTI89 / UPEC)

KEGG orthology group: K01070, S-formylglutathione hydrolase [EC: 3.1.2.12] (inferred from 99% identity to stt:t0661)

MetaCyc: 88% identical to S-formylglutathione hydrolase / S-lactoylglutathione hydrolase (Escherichia coli K-12 substr. MG1655)
Hydroxyacylglutathione hydrolase. [EC: 3.1.2.6]; S-formylglutathione hydrolase. [EC: 3.1.2.6, 3.1.2.12]

Predicted SEED Role

"S-formylglutathione hydrolase (EC 3.1.2.12)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 3.1.2.12)

MetaCyc Pathways

• formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
• superpathway of methylglyoxal degradation (6/8 steps found)
• methylglyoxal degradation I (2/3 steps found)
• methylglyoxal degradation VIII (2/3 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.6

Use Curated BLAST to search for 3.1.2.12 or 3.1.2.6

Protein Sequence (285 amino acids)

>GFF3784 S-formylglutathione hydrolase (EC 3.1.2.12) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MEMLEEHRCFGGWQQRWRHHAATLNCAMTFSIFLPPTQDNEPPPVLYWLSGLTCNDENFT
TKAGAQRIAAELGIVLVMPDTSPRGEQVADDSGYDLGHGAGFYLNATQPPWASHYRMYDY
LRDELPALIQTQFNVSDRCAISGHSMGGHGALIMALKNPGKYTSVSAFAPIVNPSRVPWG
IKALTAYLGEDESAWTEWDSCELMLASQPQDAIPVLIDQGDSDQFLADQLQPAVLAEAAR
QTAWPMTLRIQPGYDHSYYFIASFIEDHLRFHARYLRDERETSPT