Protein Info for GFF3783 in Sphingobium sp. HT1-2

Annotation: L-aspartate oxidase (EC 1.4.3.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 539 TIGR00551: L-aspartate oxidase" amino acids 6 to 526 (521 residues), 608.9 bits, see alignment E=3.4e-187 PF01266: DAO" amino acids 7 to 210 (204 residues), 29.5 bits, see alignment E=1.1e-10 PF00890: FAD_binding_2" amino acids 7 to 398 (392 residues), 335.4 bits, see alignment E=1.2e-103 PF02910: Succ_DH_flav_C" amino acids 447 to 528 (82 residues), 55.9 bits, see alignment E=8.7e-19

Best Hits

Swiss-Prot: 58% identical to NADB_VIBCH: L-aspartate oxidase (nadB) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K00278, L-aspartate oxidase [EC: 1.4.3.16] (inferred from 94% identity to sjp:SJA_C1-29110)

Predicted SEED Role

"L-aspartate oxidase (EC 1.4.3.16)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 1.4.3.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (539 amino acids)

>GFF3783 L-aspartate oxidase (EC 1.4.3.16) (Sphingobium sp. HT1-2)
MPTITHDVVIIGSGAAGLTAAINLAQDRKVVVLAKGALDGGSTNWAQGGIAAVLDAGDSF
AAHVHDTMVAGAGLNDQDTVEFVVSEAPAAIERLAELGVPFNPNEEGDGDEFGERWHLTR
EGGHSHRRIVHVDDATGHAIQVALLKAARANPNITLMEDMVAVDLITSRHGERYSGDGHV
WGVYAFNKQTGHVDTILGRATILCTGGAGRTYLFSTAPRGATGDGIAMAWRAGCRVSNME
MNQFHPTCLYNLEVKNFLITEAVRGEGGHLKLPPGVPGGGERFMPRFDARAELAPRDVVA
RAIDHEIKRLGLDYVHLDISHKPPEFVKQHFPTIYARLLDLDIDITKEPIPVVPAQHYTC
GGVIIDLDGRTDLPGLYAAGEVTESGLHGANRLASNSLLECFVFGEAAAKHIRSHWDELP
APPPIRPWDESRVTNSDEEVIVQHNWKEIRRFMWDYVGIVRTTKRLERAQHRIDLLSKEV
DEYYGNFRVTPDLIELRNLLEVARLVVRSALHRKESRGLHYTLDYPETLAQAVDTVLAP