Protein Info for GFF3774 in Sphingobium sp. HT1-2

Annotation: Cysteinyl-tRNA synthetase (EC 6.1.1.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 504 TIGR00435: cysteine--tRNA ligase" amino acids 15 to 492 (478 residues), 349.5 bits, see alignment E=1.8e-108 PF01406: tRNA-synt_1e" amino acids 28 to 342 (315 residues), 257 bits, see alignment E=2.7e-80

Best Hits

KEGG orthology group: K01883, cysteinyl-tRNA synthetase [EC: 6.1.1.16] (inferred from 89% identity to sch:Sphch_1686)

Predicted SEED Role

"Cysteinyl-tRNA synthetase (EC 6.1.1.16)" in subsystem Conserved gene cluster possibly involved in RNA metabolism (EC 6.1.1.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (504 amino acids)

>GFF3774 Cysteinyl-tRNA synthetase (EC 6.1.1.16) (Sphingobium sp. HT1-2)
MAGAMSQDQHSDAPLRLFNSLTRSFEPFSPIEPNHARVYSCGPTVYNYAHIGNLRAYVFV
DTLRRTLLWKGLDVTQIINITDVGHLTSDADAGDDKMEAAARASAKSIWDIAAHYTEAFK
ANIADLNIMAPSEWTVATDYVPQMIEFAQKIADAHCYELESGLYFDSSTVPNYGALAGGR
DDAAYARIDPVAGKRNPSDFAIWRKSPPGEQRQMEWDSPWGKGAPGWHLECSVMSQARLG
QPFDIHTGGIDHREIHHPNEIAQNQAYCGCTDAQPDFTGARWWMHNNFLVDRQGKMSKSK
GGFTTLFSLVDAGVHPIAYRLLCLGAHYRSELEFSADNLAAALTRLKRLVMGVEGLKGRA
EGITWQSVTLDYLRANLHPKLVPLLEQFDAAIADDLMTPRALPLLEEAIAMKKVPVDEKL
CLIAAFDQALGLRLLDLTRAELRVRPKDAQITPEEIEAELERRTAARAEKDFALSDEIRD
ALIARGVEVMDGDPLRWDWRLTLA