Protein Info for GFF3772 in Sphingobium sp. HT1-2

Annotation: D-lactate dehydrogenase (EC 1.1.1.28)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 5 to 327 (323 residues), 111.6 bits, E=2.3e-36
• PF02826: 2-Hacid_dh_C
  • amino acids 108 to 296 (189 residues), 164.9 bits, E=1.3e-52

Best Hits

Swiss-Prot: 57% identical to DDH1_SCHPO: 2-hydroxyacid dehydrogenase homolog 1 (SPAC186.07c) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K03778, D-lactate dehydrogenase [EC: 1.1.1.28] (inferred from 72% identity to cse:Cseg_3088)

MetaCyc: 52% identical to D-lactate dehydrogenase (Escherichia coli K-12 substr. MG1655)
D-lactate dehydrogenase. [EC: 1.1.1.28]

Predicted SEED Role

"D-lactate dehydrogenase (EC 1.1.1.28)" in subsystem Fermentations: Lactate or Fermentations: Mixed acid (EC 1.1.1.28)

MetaCyc Pathways

• heterolactic fermentation (16/18 steps found)
• superpathway of glucose and xylose degradation (14/17 steps found)
• mixed acid fermentation (13/16 steps found)
• L-alanine degradation II (to D-lactate) (3/3 steps found)
• pyruvate fermentation to (R)-lactate (1/1 steps found)
• superpathway of fermentation (Chlamydomonas reinhardtii) (6/9 steps found)
• vancomycin resistance I (1/3 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (4/8 steps found)
• L-alanine degradation VI (reductive Stickland reaction) (2/6 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (4/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.28

Use Curated BLAST to search for 1.1.1.28

Protein Sequence (337 amino acids)

>GFF3772 D-lactate dehydrogenase (EC 1.1.1.28) (Sphingobium sp. HT1-2)
MRIAIFGTKGYDRHFLSAANQGHELHFLEPRLDADTASLGEGFEAVCVFVNDRLDAAVLA
KLAAGGTRLIALRCAGYNNVDLVAAADLGLTVVRVPAYSPHAVAEFTVALLMALDRRIHR
AWARVRENNFALDGLIGRNLHGRVAGVIGTGRIGALVARTLKLGFGCEVLASDVIEDPDL
LAMGVRYVPPRDLLEQVDIVSLHCPLTPDTRHIINADAIGRARDGLVIVNTSRGALIDTQ
ALVAGLKSRKIGGVALDVYEQEADLFFEDLSNEIIEDDLFQRLLTFPNVLVTGHQAFLTE
EALASIAETTMTNISDFAAGRPLANRVDPQAAIAPRG