Protein Info for GFF3771 in Xanthobacter sp. DMC5

Annotation: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 442 TIGR00612: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase" amino acids 26 to 416 (391 residues), 427.3 bits, see alignment E=2.5e-132 PF04551: GcpE" amino acids 31 to 416 (386 residues), 373.2 bits, see alignment E=6.1e-116

Best Hits

Swiss-Prot: 79% identical to ISPG_RHILO: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K03526, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase [EC: 1.17.7.1] (inferred from 92% identity to xau:Xaut_1889)

Predicted SEED Role

"1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1)" in subsystem Isoprenoid Biosynthesis (EC 1.17.7.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.7.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (442 amino acids)

>GFF3771 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (Xanthobacter sp. DMC5)
MTSAETLTSAAPVVPVELLEAGPARRHATVGVPVGRVMVGGGAPVVVQSMTNTDTADIAG
TVRQVAALARAGSEIVRITVDRDEAAAAVPHIYEKLLAQGIDVPLVGDFHYIGHKLLADH
PACAEALAKYRINPGNVGFGDKKDRQFAAIVETAIRHDKPVRIGANWGSLDGELLTRLMD
ENARLPRPAEARAVTREALVRSALLSAALAEEIGLGRNRIILSAKVSAVQDLIAVYSELA
RRSDYALHLGLTEAGMGSKGIVASTAAMGVVLQQGIGDTIRVSLTPEPNGDRTREVQVAQ
EILQTMGLRTFVPLVAACPGCGRTTSTVFQELAQSVQDMIRTRMPVWKAQYPGVETLNVA
VMGCIVNGPGESKHADIGISLPGTGETPSAPVFIDGKKVATLRGANIQEEFRQMVEAYVA
KRFGIGGGAGVAESGTPADAAE