Protein Info for GFF3765 in Sphingobium sp. HT1-2

Annotation: Na+/H+-dicarboxylate symporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 448 transmembrane" amino acids 21 to 40 (20 residues), see Phobius details amino acids 52 to 75 (24 residues), see Phobius details amino acids 89 to 111 (23 residues), see Phobius details amino acids 141 to 157 (17 residues), see Phobius details amino acids 163 to 181 (19 residues), see Phobius details amino acids 200 to 222 (23 residues), see Phobius details amino acids 234 to 261 (28 residues), see Phobius details amino acids 273 to 287 (15 residues), see Phobius details amino acids 318 to 337 (20 residues), see Phobius details amino acids 344 to 388 (45 residues), see Phobius details PF00375: SDF" amino acids 21 to 413 (393 residues), 358 bits, see alignment E=3.4e-111

Best Hits

Swiss-Prot: 73% identical to DCTA_PARDP: C4-dicarboxylate transport protein (dctA) from Paracoccus denitrificans (strain Pd 1222)

KEGG orthology group: K11103, aerobic C4-dicarboxylate transport protein (inferred from 87% identity to sch:Sphch_2785)

MetaCyc: 62% identical to C4 dicarboxylate/orotate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-121; TRANS-RXN-121A; TRANS-RXN-121C; TRANS-RXN-122A; TRANS-RXN0-451; TRANS-RXN0-517; TRANS-RXN0-553

Predicted SEED Role

"C4-dicarboxylate transport protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (448 amino acids)

>GFF3765 Na+/H+-dicarboxylate symporter (Sphingobium sp. HT1-2)
MLPTPSTPTEPVRPLPFYRHLYFQVLVAIALGVLLGHYFPATGEAMKPLGDAFIALVRMI
IAPVIFLTIVTGIAGMKELGAIGRVAAKAFAYFLTFSTLALIVGLIIANVVQPGAGLNID
PATLDASKIADYAQQAHDRTLVAFLLNVIPATLVSAVADGHNILQVLFVAILFGIALSMI
GEKAEPLMTVLESASLAIFKLVSFLMKAAPFGAFGAMAFTVAKYGVGTLANLAGLVATFY
LTSLLFVLVVLGTVAWFAGFNILHLIRYLRAELLLVLGTSSSEAALPSLIEKMEQAGCRK
SVVGLVVPTGYSFNLDGTNIYMTLAALFIAQATNVHLSLEEQILLLLVAMLSSKGAAGVT
GAGFITLAATLSIVPSVPVAGMTLILGVDRFMSECRSLTNFVGNAVATVVVSVWEKGLDR
ERFAAAMAGKPLPPLGQGAEEVAAEALS