Protein Info for GFF3758 in Xanthobacter sp. DMC5

Annotation: putative peptidoglycan D,D-transpeptidase FtsI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 648 transmembrane" amino acids 106 to 127 (22 residues), see Phobius details PF03717: PBP_dimer" amino acids 144 to 254 (111 residues), 40 bits, see alignment E=5.7e-14 PF00905: Transpeptidase" amino acids 316 to 600 (285 residues), 193.4 bits, see alignment E=5.3e-61

Best Hits

KEGG orthology group: K03587, cell division protein FtsI (penicillin-binding protein 3) [EC: 2.4.1.129] (inferred from 88% identity to xau:Xaut_1850)

Predicted SEED Role

"Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)" in subsystem Bacterial Cell Division or Bacterial Cytoskeleton or Flagellum in Campylobacter or Peptidoglycan Biosynthesis (EC 2.4.1.129)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129

Use Curated BLAST to search for 2.4.1.129

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (648 amino acids)

>GFF3758 putative peptidoglycan D,D-transpeptidase FtsI (Xanthobacter sp. DMC5)
MADLSSISARLARLWDGIVATVGPVLRDADAACAVLLDRLGAGSKAAGRATARGSARAAL
ASWNVLWPFTVRLGYLAWRGIRHVVRGLFGLDRGDPDTVARGRIRLVMLAFCCLFVAIAV
QLSHLAMTADGGGGRGRGSDAVASARPDIIDRNGDIVAIDVKSPSLFAEPRRLIDPDEAL
EGLLKVLPDLDVEEARKRLNSGKGFAWLKREITPAQMRAIHRLGFPGIGFLKENRRIYPD
GPIISHVMGGVNVDNQGISGIEKWIDSRGLAELHLAGFATDRQQEPVQLSMDVRVQHAVR
DELVKAKNKFQALHAMAVVTEVNTGEIISLVSIPDFDPNTSGNPKDDAYLNRLTTGVYEM
GSTFKALSFAMALDSGKIGLNSTFDARGALHFGKFAIHDYHAENRVLTVPEIFLVSSNVG
TAKMVLSLGVEAHKAFLKKMGQLDRLKTELPESSEPIVPKRWGELNSATIAFGHGLSVAP
LQAVMAVNSMVNGGFLIPPTFIKRTPEEAQKVAVRVLKPETSDKMRYIMRLNAEKGTATK
AEVPGFLVGGKTGTAEKVVNGRYAKNKLLTSFTAVFPMDKPRYLVLVMLDEPKATSETYG
FATSGWNAAPTAGKIVERIAPMLNIAPRQNLPNAEQLLRMPLKVADRN