Protein Info for GFF3756 in Xanthobacter sp. DMC5

Annotation: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alani ne ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 37 to 108 (72 residues), 33.5 bits, E=7e-12
• TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
  • amino acids 41 to 472 (432 residues), 367 bits, E=6.8e-114
• PF08245: Mur_ligase_M
  • amino acids 121 to 311 (191 residues), 148.1 bits, E=4.9e-47
• PF02875: Mur_ligase_C
  • amino acids 339 to 460 (122 residues), 65.5 bits, E=1.3e-21

Best Hits

KEGG orthology group: K01929, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase [EC: 6.3.2.10] (inferred from 86% identity to xau:Xaut_1848)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.10

Protein Sequence (486 amino acids)

>GFF3756 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alani ne ligase (Xanthobacter sp. DMC5)
MNSGTGMGETEGRVLHTVAEMAAAMGAEVRGTPRDATGISIDSRSLAPGDAFFAIAGENS
DGHAYVGKALEAGASACVVARDKLDQFPADAPLLVVDDVLAGLTDLARASRARSRAGIVA
VTGSVGKTTTKEALRIALGADGETHASAASYNNHWGVPLSLARLPREARYGVFEIGMNHP
GEITPLVKLVRPQVAIITTVEPVHIAQFSGIDEIADAKAEIFQGLEPGGAVVLNLDNPLF
PRLKAAAEALGIDRIVTFGETAGADVRLKEAALQSYCSVGVADVMGTPVTFKVGMPGRHI
VQNMLAVLAAAKLVGADLALVGLALSRMKAPPGRGVALRLQVKGGQATLLDESYNANPAS
MRAALDVLSRTPVGPRGRRIAVLGDMLELGPDGEAHHAELAEAIRTAKIDQVFCCGPQMH
ALWQALPSERRGGYAIHASHLEPMVASAIRGGDTLMVKGSNGSRMGPLVKSLAERFRAAE
DALLEG