Protein Info for PS417_19225 in Pseudomonas simiae WCS417

Annotation: FAD-dependent cmnm(5)s(2)U34 oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 666 PF05430: Methyltransf_30" amino acids 112 to 232 (121 residues), 135.3 bits, see alignment E=1.7e-43 TIGR03197: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain" amino acids 262 to 657 (396 residues), 459.5 bits, see alignment E=5.4e-142 PF01266: DAO" amino acids 263 to 628 (366 residues), 178.5 bits, see alignment E=4.4e-56 PF13450: NAD_binding_8" amino acids 265 to 300 (36 residues), 25 bits, see alignment 2.9e-09

Best Hits

Swiss-Prot: 82% identical to MNMC_PSEF5: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: None (inferred from 85% identity to pfs:PFLU4318)

Predicted SEED Role

"5-methylaminomethyl-2-thiouridine-forming enzyme mnmC"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7TZI5 at UniProt or InterPro

Protein Sequence (666 amino acids)

>PS417_19225 FAD-dependent cmnm(5)s(2)U34 oxidoreductase (Pseudomonas simiae WCS417)
MNPVTHAQLDWDDQGRPRSRVFDDVYFSDKSGLEETRYVFLEQNRLQERFAALPVGGRLV
IGETGFGTGLNFLCAWQLFEQHAVAGAHLHFVSVEKYPLSRADLQRALALWPELQPLAAQ
LLAQYIAIHQGFQRLVLDNGRVTLTLLIGDALEQLPQLDAQVDAWFLDGFAPAKNPEMWT
AELFAELARLAAPGSTISTFTSTGWVRRLINAAGFKMKRTPGIGHKWEILRGEFLGWPAD
APAPSAAKPWFARPAALAGERHALVIGGGLAGCATASSLAARGWQVSLLERHASLAQEAS
GNPQGVLYLKLSAHGTALSQLIVAGFGHTRRLLEHLHRGVDWDGCGVLQLAFNAKEAERQ
AQLAHAFAPDLLHLLDRDQAQQQAGIRLAQGGLFFPEGGWVHPPALCQWQAAHPLIKVLT
HHEALELHRVDDQWQARDGDRVLASATVVVLAGAADIKRFALSADLPLKRIRGQITRLAQ
TTASAALATVVCAEGYVAPARRGEHTLGASFDFNNDDLTPTVAEHAGNLAMLREISEDLV
QRLGANQLVPEQLEGRAAFRCTSPDYLPIVGPLADTEAFQRTYAVLSKDARQVPDMPCPW
LPGVYINSGHGSRGLITAPLCGELLAAWLNDEPLPVPASVAEACHPNRFALRALVRGKST
VNKEWH