Protein Info for GFF3753 in Xanthobacter sp. DMC5

Annotation: UDP-N-acetylmuramoylalanine--D-glutamate ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 68% identical to MURD_NITWN: UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) from Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)

KEGG orthology group: K01925, UDP-N-acetylmuramoylalanine--D-glutamate ligase [EC: 6.3.2.9] (inferred from 84% identity to xau:Xaut_1845)

Predicted SEED Role

"UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.9)

MetaCyc Pathways

peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (518 amino acids)

>GFF3753 UDP-N-acetylmuramoylalanine--D-glutamate ligase (Xanthobacter sp. DMC5)
MIPVTVFKDATVALFGLGGSGLATAEALKAGGATVVAYDDSEASRRKAGEAGILVQDLHD
IDWTIVQALVLAPGVPLTHPEPHWSVKLAQAAGVEIIGDIELFCRERQAIARRSPFVAIT
GTNGKSTTTALITHILRVAGRDVQMGGNIGTAILSLAPPKPGRVHVVECSSYQIDLAPGL
DPSVGVMLNITPDHLDRHGTIENYAAVKERLVTGVEQTGTAVVGVDDDFSAAMADRAERA
GKHVVRISVRRPLPDGIWREGETLVVSEGGAEVFRLPLGGIGSLRGAHNAQNAAAAYAAC
RALGVAPEVIAVAMKRFPGLAHRMEEVGNKGKVLFVNDSKATNADAAERALTSFTDIFWI
AGGKPKAGGIGPLAPFFDHIAKAYLIGEAAQEFAATLADKVPHEIVGTLDKAVEAAARDA
EAAGRADAVVLLSPACASFDQYPNFEVRGDAFRTLVQALPGIEARTRSTTPVAQKEPRKG
GGHPHGNPHGNPHGGGHPHGMGGHPGGHPGGHPGKGHP