Protein Info for GFF375 in Sphingobium sp. HT1-2

Annotation: Peptidoglycan lipid II flippase MurJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 524 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 51 to 69 (19 residues), see Phobius details amino acids 90 to 111 (22 residues), see Phobius details amino acids 129 to 155 (27 residues), see Phobius details amino acids 162 to 183 (22 residues), see Phobius details amino acids 195 to 214 (20 residues), see Phobius details amino acids 235 to 263 (29 residues), see Phobius details amino acids 280 to 299 (20 residues), see Phobius details amino acids 320 to 339 (20 residues), see Phobius details amino acids 359 to 379 (21 residues), see Phobius details amino acids 391 to 409 (19 residues), see Phobius details amino acids 415 to 436 (22 residues), see Phobius details amino acids 452 to 469 (18 residues), see Phobius details amino acids 485 to 511 (27 residues), see Phobius details TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 2 to 508 (507 residues), 410.1 bits, see alignment E=7e-127 PF03023: MurJ" amino acids 29 to 471 (443 residues), 384.1 bits, see alignment E=8.5e-119 PF13440: Polysacc_synt_3" amino acids 225 to 344 (120 residues), 26.7 bits, see alignment E=3.3e-10

Best Hits

KEGG orthology group: K03980, virulence factor (inferred from 88% identity to sjp:SJA_C1-08280)

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (524 amino acids)

>GFF375 Peptidoglycan lipid II flippase MurJ (Sphingobium sp. HT1-2)
MKLVKALGSVGGLTLASRVLALVRDSLAARYVGAGFASDAFNGVAFRLPNMFRALFAEGA
FSAAFIPLFNKKAAGPGGMADGYHFAERALAVLLPVLILFTIVLLAAAWPITWALSGGFS
RQNPTPDQFAFAVTLSRITIPYLALISLASLLGGILNSLDKFWVNAAAPILLNVAMIAGL
WFFHGADEYETARVQAMSVTVGGALQLLWLIWACKRAGVSMKLKRPRLDADVRELLRLIV
PAAAGAGASQINLLISTALSGWLLASGSITYIYYADRLNQLPLGLIGIGLGTILLPTISR
MLSKGEDQAAMETQNRGIELALFLTLPATVAFITVAEPIVRGLFQYGRFSAEDAMRCGWA
LSAFSIGLPSYVLVKVLTPGYYARGDTKTPVRYAMLSIAINIVGNLAMIPTLGHIGPPLA
TALSSTVNVAMLYRTLVARGHFTADPQLRRRIPRLAVAAVVMGVALWAGEDLLDPMLSGP
MVGRYIGLALLVGAGVALYGLACFVTGAYRLSDLKALMRRRGTT