Protein Info for GFF3743 in Xanthobacter sp. DMC5

Annotation: Ribosomal RNA small subunit methyltransferase G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 218 PF02527: GidB" amino acids 31 to 193 (163 residues), 117.5 bits, see alignment E=2.4e-38 TIGR00138: 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG" amino acids 32 to 189 (158 residues), 108.1 bits, see alignment E=2e-35

Best Hits

Swiss-Prot: 53% identical to RSMG_METC4: Ribosomal RNA small subunit methyltransferase G (rsmG) from Methylobacterium extorquens (strain CM4 / NCIMB 13688)

KEGG orthology group: K03501, ribosomal RNA small subunit methyltransferase G [EC: 2.1.1.170] (inferred from 73% identity to xau:Xaut_1835)

Predicted SEED Role

"rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.170

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (218 amino acids)

>GFF3743 Ribosomal RNA small subunit methyltransferase G (Xanthobacter sp. DMC5)
MKGAGAGGDDAARAAVEGLVSRETFRCLDLIAELLRKWQKTINLVAPATLPEVWSRHIAD
SLQLINHVPADVARWVDLGSGGGFPGLVIAAVLAERSGADVHLVESDTRKSAFLREAARV
AELPVRVHAQRIEQVAQTLAPGTQVVSARALAPLPKLLELAEPFLSAGALGVFPKGRDAE
RELTEARKGWTLDCDLRASVSDREARVLLVRSARRSSK