Protein Info for GFF3728 in Sphingobium sp. HT1-2

Annotation: Flagellar M-ring protein FliF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 582 transmembrane" amino acids 52 to 71 (20 residues), see Phobius details amino acids 472 to 494 (23 residues), see Phobius details PF01514: YscJ_FliF" amino acids 72 to 245 (174 residues), 180.8 bits, see alignment E=2.3e-57 TIGR00206: flagellar M-ring protein FliF" amino acids 76 to 576 (501 residues), 234.6 bits, see alignment E=1.5e-73 PF08345: YscJ_FliF_C" amino acids 275 to 453 (179 residues), 121.2 bits, see alignment E=5.1e-39

Best Hits

KEGG orthology group: K02409, flagellar M-ring protein FliF (inferred from 86% identity to sjp:SJA_C1-30830)

Predicted SEED Role

"Flagellar M-ring protein FliF" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (582 amino acids)

>GFF3728 Flagellar M-ring protein FliF (Sphingobium sp. HT1-2)
MSENALTIDGGSAAAPALPAVTGASFGNAKGVDALKARFAGFIKQPAVAKSLPLLGLLGT
VAVAGAAWLALREPPQRDLFRGLPDGDKSAVAQVLDQNGIKYDFDNAGGMTVGEGDYFKA
KMMLAAQGLPKSAPDGNSMIDSLPMGASRAVENEKLRGARETDLARTIEAIDSVESAKVH
LAVEAPSVFLRERTKPSASVMLRLSQGRTLTDAQVSAIVHLVASSIPELNPEDISVVDQN
GRLLSNNDGNAADDRQLAIQTRIEDRYRQSVVALLTPILGDGKFSAEVHAELNFAERQAT
RESFPQDEARLRAEQGSWSSDERGQKAGEASGIPGALSNQAPVNPTVTQTNPNGQAVTQG
QTAEQQQPATPGLPPMKTEETFNRSFELGREVSVTKDAVGTVKRLSVAVALDTGADGKPR
SAQEIAALEALVKGAVGFDQARGDVVALSARSFAKAEEVKAPWYEADWMSPLIRNVSALL
VALLVIFGIGRPLLKRRAAAQEAKAVETAEAGQRLGREISGELTKQATENPDPSRPVTLD
MISSTYDYAQRADLIRNFVKQDPDRAALVVRDLLKEGKKENA