Protein Info for GFF3721 in Sphingobium sp. HT1-2

Annotation: Flagellar biosynthesis protein FlhA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 705 transmembrane" amino acids 15 to 36 (22 residues), see Phobius details amino acids 42 to 61 (20 residues), see Phobius details amino acids 73 to 93 (21 residues), see Phobius details amino acids 114 to 137 (24 residues), see Phobius details amino acids 205 to 226 (22 residues), see Phobius details amino acids 238 to 264 (27 residues), see Phobius details amino acids 277 to 299 (23 residues), see Phobius details amino acids 304 to 322 (19 residues), see Phobius details TIGR01398: flagellar biosynthesis protein FlhA" amino acids 18 to 686 (669 residues), 841 bits, see alignment E=3.3e-257 PF00771: FHIPEP" amino acids 27 to 676 (650 residues), 795.3 bits, see alignment E=2.5e-243

Best Hits

Swiss-Prot: 49% identical to FLHA_SALTY: Flagellar biosynthesis protein FlhA (flhA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02400, flagellar biosynthesis protein FlhA (inferred from 91% identity to sjp:SJA_C1-30900)

Predicted SEED Role

"Flagellar biosynthesis protein FlhA" in subsystem Flagellar motility or Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (705 amino acids)

>GFF3721 Flagellar biosynthesis protein FlhA (Sphingobium sp. HT1-2)
MTPTQVKAKIWMSAAKGAVLPFATLMVVLFMMVPVPAVMLDIGFITNIMISLAVLMVALN
AAKPLDFSSFPTVLLFATLLRLALNVASTRVVLVSGHEGSDAAGQVIEAFGHFLIGGDYV
VGIFVFAILMIINLVVITKGAGRVSEVSARFTLDALPGKQMAIDADLNAGLMTPEEAKLR
RAEVATEADFYGSMDGASKFVKGDAIAGILILAINIIGGIILGVVSHGLAMGDAAQTYIL
LAIGDALVAQIPALLLSIAAASIVTRVKSEQDLSAQVAGQFGTGKAWVPVAAILGFLGIL
PGMPHLIILTAAAIAGGIAWQLRKAGQRKAAEPEPVAPPPNPAIIEWNDVSDGAILGLEI
GYGLIGLVDERKGAPLMARITGIRRQLSKELGFVVPMVRVKDNLALEPNQYRITIAGVVV
GEDDIWPDDLLALDSGMLEGTVAGRPAKDPTFGLDAVWISPAKRSEAVVAGYTVVDPPTV
VATHLNQLIAMNAAEMFGLDEARKLLDNLKDVAPQLVDGLTPGTLSLTQISALCRALLSE
GIALKDFRRICEAMVDAARPDMNHEQLVEAVRQRIGALIIQGLVPVKMPLPVITLDGDLE
AMLAQAMRVAGDAKHPIEPSLGNRIVESVVHAARPMLGQARNFAIVTSPVARRALARLFK
PHLPETPVLSFLEIPDGKGVEVVAVVGGEQRPTPRHDPLPRERVA