Protein Info for GFF3708 in Sphingobium sp. HT1-2

Annotation: Flagellar L-ring protein FlgH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 226 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF02107: FlgH" amino acids 47 to 225 (179 residues), 197.3 bits, see alignment E=7.8e-63

Best Hits

Swiss-Prot: 46% identical to FLGH_BURM1: Flagellar L-ring protein (flgH) from Burkholderia multivorans (strain ATCC 17616 / 249)

KEGG orthology group: K02393, flagellar L-ring protein precursor FlgH (inferred from 82% identity to sjp:SJA_C1-31030)

Predicted SEED Role

"Flagellar L-ring protein FlgH" in subsystem Flagellar motility or Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (226 amino acids)

>GFF3708 Flagellar L-ring protein FlgH (Sphingobium sp. HT1-2)
MRALSTSLVALAMVSMAASPAFAAKKKREVEREYYAPTVIAQPQAPQANGSIFQVSTGYT
PLTSGARAGNVGDIITIVLVERTQATKSNSADTNRSGSIGLNPPTTGPLSKLFSSSDIAM
GGNNTFTGKGAATQSNALNGEITVTVAAAYPNGTMLVKGEKALTLNRGDEFIQISGLVRQ
ADIGPDNRILSTRVADAKIIYTGKGEIARASRQGWLQRFFSMISPF