Protein Info for GFF3701 in Sphingobium sp. HT1-2

Annotation: L-Proline/Glycine betaine transporter ProP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 515 transmembrane" amino acids 61 to 79 (19 residues), see Phobius details amino acids 85 to 109 (25 residues), see Phobius details amino acids 121 to 143 (23 residues), see Phobius details amino acids 151 to 175 (25 residues), see Phobius details amino acids 190 to 209 (20 residues), see Phobius details amino acids 221 to 240 (20 residues), see Phobius details amino acids 278 to 299 (22 residues), see Phobius details amino acids 315 to 335 (21 residues), see Phobius details amino acids 344 to 363 (20 residues), see Phobius details amino acids 369 to 391 (23 residues), see Phobius details amino acids 405 to 428 (24 residues), see Phobius details amino acids 438 to 456 (19 residues), see Phobius details PF00083: Sugar_tr" amino acids 53 to 258 (206 residues), 113.9 bits, see alignment E=9e-37 amino acids 267 to 464 (198 residues), 59.8 bits, see alignment E=2.4e-20 PF07690: MFS_1" amino acids 61 to 414 (354 residues), 119 bits, see alignment E=2.2e-38

Best Hits

Swiss-Prot: 54% identical to OUSA_DICD3: Glycine betaine/proline/ectoine/pipecolic acid transporter OusA (ousA) from Dickeya dadantii (strain 3937)

KEGG orthology group: K03762, MFS transporter, MHS family, proline/betaine transporter (inferred from 81% identity to smt:Smal_2178)

Predicted SEED Role

"L-Proline/Glycine betaine transporter ProP" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (515 amino acids)

>GFF3701 L-Proline/Glycine betaine transporter ProP (Sphingobium sp. HT1-2)
VPAWTGREINVSTSAAHAARHTHFGWFKRRRDLEVDDVTVVDRSLLNRAVGAAALGNAME
WFDFGVYGYIAVTLGHVFFPSSDPALQLIATFATFTVAFLVRPLGGLVFGPLGDRYGRHK
ILAMTMILMAVGTFSIGLIPSYAQIGIGAPLMLLAARLVQGFSTGGEYGGAATFIAEYST
DRKRGLMGSWLEFGTLGGYIAGAGTVTALQMSLSETQMLDWGWRIPFLVAGPLGLLGLYM
RLKLEETPAFQAYSEQIDARESERPGLGTLFRVHWRQLLKCVGLVLVFNVTDYMLLTYMP
NYLSVTMGYAETKGLLLIIIVMLVMMPLNVVGGLFSDRLGRRPMIIGACVALMLLSVPCM
LLIGTGNDALIFLGLMLLGIALVCFTSSMPSTLPALFYTPVRYSALSIAFNISVSLFGGT
TPLITAWLVQKTGDPLVPAYYLMGAALIGLLTMLTVRETAGMPLRGSPPAVANEAEAIAL
VESGEPVTVDKALPDLPPVAPVAVETPAMIRPLAS