Protein Info for GFF3695 in Xanthobacter sp. DMC5

Annotation: Malate synthase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF20656: MS_N
  • amino acids 14 to 74 (61 residues), 79.5 bits, E=1.6e-26
• TIGR01344: malate synthase A
  • amino acids 27 to 535 (509 residues), 853.8 bits, E=1.7e-261
• PF01274: MS_TIM-barrel
  • amino acids 166 to 410 (245 residues), 352.1 bits, E=1.8e-109
• PF20659: MS_C
  • amino acids 417 to 534 (118 residues), 137.3 bits, E=5.1e-44

Best Hits

Swiss-Prot: 61% identical to MASY_MYXXD: Malate synthase (mls) from Myxococcus xanthus (strain DK 1622)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 82% identity to azc:AZC_0078)

MetaCyc: 56% identical to malate synthase A (Escherichia coli K-12 substr. MG1655)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• glyoxylate cycle (5/6 steps found)
• glycolate and glyoxylate degradation II (2/2 steps found)
• chitin deacetylation (3/4 steps found)
• superpathway of glycol metabolism and degradation (5/7 steps found)
• D-xylose degradation IV (3/7 steps found)
• L-arabinose degradation IV (3/8 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (6/14 steps found)
• superpathway of pentose and pentitol degradation (9/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Protein Sequence (536 amino acids)

>GFF3695 Malate synthase A (Xanthobacter sp. DMC5)
MSSPALKTAPAAGIEITGALGPRFDEILTPEALAFVADLHRAFNPTRKALLARRTERQVK
FDAGELPDFLPETKAVRDGDWTIADLPADLLDRRVEITGPVDRKMIVNALNSGAKVFMAD
FEDASSPVWENMIEGQINLKDRWVGAIDFVDANNGKSYKLGATPAVLIIRPRGWHLPEAH
VLVDGEEISGSLFDFGLYVFHNAKAALAQGSGPYFYLPKTESHLEARLWNEVFVAAQKAL
GLPVGTIKATVLIETLPAVFEMDEILFEMKDHIAGLNLGRWDYIFSFIKRLGKNDRFLTP
DRSRMVMSEAFLKAYGILLVRTCHRRNAFAMGGMAAQIPVKGDPEANAAAFAKVKADKER
EANSGCDGTWVAHPDLVPVAMEVFNRLMPAANQRDAKVSDVPVSRDDLLEVHAGTRSEAG
LRENIRVGVQYIEAWLRGRGAVPLYNLMEDAATAEISRAQVWQWIHLGATLDDGRAVTPE
LFRALLDDEMANLRSVLGPEVYDSGRFPEAIKLFSDMTLADSFEEFLTLPAYRLIA