Protein Info for PS417_18895 in Pseudomonas simiae WCS417

Annotation: glucarate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03247: glucarate dehydratase
  • amino acids 2 to 441 (440 residues), 869.6 bits, E=1.9e-266
• PF13378: MR_MLE_C
  • amino acids 184 to 401 (218 residues), 163.5 bits, E=3.1e-52

Best Hits

Swiss-Prot: 80% identical to GUDD_PSEPU: Glucarate dehydratase (gudD) from Pseudomonas putida

KEGG orthology group: K01706, glucarate dehydratase [EC: 4.2.1.40] (inferred from 96% identity to pfs:PFLU4261)

MetaCyc: 80% identical to D-glucarate dehydratase subunit (Pseudomonas putida)
Glucarate dehydratase. [EC: 4.2.1.40]

Predicted SEED Role

"Glucarate dehydratase (EC 4.2.1.40)" in subsystem D-galactarate, D-glucarate and D-glycerate catabolism (EC 4.2.1.40)

MetaCyc Pathways

• D-glucarate degradation II (3/3 steps found)
• superpathway of D-glucarate and D-galactarate degradation (4/5 steps found)
• D-glucarate degradation I (3/4 steps found)
• superpathway of microbial D-galacturonate and D-glucuronate degradation (13/31 steps found)

KEGG Metabolic Maps

• Ascorbate and aldarate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.40

Sequence Analysis Tools

See A0A1N7UI75 at UniProt or InterPro

Protein Sequence (441 amino acids)

>PS417_18895 glucarate dehydratase (Pseudomonas simiae WCS417)
MTTPVVTHFQVIPVAGHDSMLLNLSGAHGPYFTRNIVILKDSSGNTGVGEVPGGERIRET
LEDARSLVIGQPIGQYQRILNQMRSTFASRDAAGRGLQTFDLRITIHAVTAMEAALLDLL
GQFLDVPVAALLGEGQQRDAVKMLGYLFYVGDRSATPLAYRNEADSDDEWFRLRHETALT
ADAVVRLAEAAKAKYGFNDFKLKGGVLSGDEEIQAVTALAERFPDARITLDPNGAWSLKE
AIRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLKTATNMIATDWREMGHAIQL
QSVDIPLADPHFWTLQGSVRVAQMCHEWGLTWGSHSNNHFDISLAMFTQVAAAAPGDITA
IDTHWIWQDGQHLTKAPLKIEGGYVKVPNKPGLGVEIDMDAVAKAHEVYKGMGLGARDDS
VAMQFLIPGWRFNNKQPCLVR