Protein Info for GFF369 in Xanthobacter sp. DMC5

Annotation: ADP-L-glycero-D-manno-heptose-6-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 337 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF04321: RmlD_sub_bind" amino acids 12 to 291 (280 residues), 42 bits, see alignment E=1.6e-14 TIGR02197: ADP-glyceromanno-heptose 6-epimerase" amino acids 12 to 329 (318 residues), 361.5 bits, see alignment E=1.8e-112 PF01073: 3Beta_HSD" amino acids 13 to 191 (179 residues), 28.6 bits, see alignment E=1.9e-10 PF01370: Epimerase" amino acids 13 to 258 (246 residues), 140.2 bits, see alignment E=1.9e-44 PF16363: GDP_Man_Dehyd" amino acids 13 to 308 (296 residues), 75.7 bits, see alignment E=1.2e-24

Best Hits

Swiss-Prot: 40% identical to HLDD_KLEPN: ADP-L-glycero-D-manno-heptose-6-epimerase (hldD) from Klebsiella pneumoniae

KEGG orthology group: K03274, ADP-L-glycero-D-manno-heptose 6-epimerase [EC: 5.1.3.20] (inferred from 91% identity to xau:Xaut_3772)

Predicted SEED Role

"ADP-L-glycero-D-manno-heptose-6-epimerase (EC 5.1.3.20)" in subsystem LOS core oligosaccharide biosynthesis (EC 5.1.3.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.20

Use Curated BLAST to search for 5.1.3.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (337 amino acids)

>GFF369 ADP-L-glycero-D-manno-heptose-6-epimerase (Xanthobacter sp. DMC5)
MTRSVALSRDLLLVTGGAGFIGSNIARAAAEDGYRVVVADWLEDGPKWRNISDIPLHDVI
RPETINDFVEKEGGRLAAVVHMGAISATTERDADKIVARNIRATLDLWDLCAQKALGLIF
ASSAATYGDGTQGFRDSEAGDDLAALAPLNPYGWSKLFVDRRIMADITEGRPRPPQFAGL
RFFNVYGPGEAHKGDMRSVVHKIYPNAEAGESVTLFKSHDPRYTDGGQLRDFVYVGDCVE
VVRWLLESPDVSGIFNVGTGEARSFADLATAVFTALGKAPNITYVDMPESLQKAYQYFTQ
ADVSKLRAAGFTRPFTSLEDGVSAYVNRHLKPRNTAG