Protein Info for GFF3684 in Variovorax sp. SCN45

Annotation: ATP synthase alpha chain (EC 3.6.3.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 537 TIGR00962: ATP synthase F1, alpha subunit" amino acids 3 to 496 (494 residues), 790.8 bits, see alignment E=2.6e-242 PF02874: ATP-synt_ab_N" amino acids 27 to 97 (71 residues), 53.1 bits, see alignment E=5.5e-18 PF00006: ATP-synt_ab" amino acids 154 to 380 (227 residues), 267.9 bits, see alignment E=9.7e-84 PF00306: ATP-synt_ab_C" amino acids 387 to 495 (109 residues), 133.4 bits, see alignment E=9.2e-43

Best Hits

Swiss-Prot: 96% identical to ATPA_VEREI: ATP synthase subunit alpha (atpA) from Verminephrobacter eiseniae (strain EF01-2)

KEGG orthology group: K02111, F-type H+-transporting ATPase subunit alpha [EC: 3.6.3.14] (inferred from 97% identity to vpe:Varpa_5510)

MetaCyc: 70% identical to ATP synthase F1 complex subunit alpha (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP synthase alpha chain (EC 3.6.3.14)" in subsystem F0F1-type ATP synthase (EC 3.6.3.14)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.14

Use Curated BLAST to search for 3.6.3.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (537 amino acids)

>GFF3684 ATP synthase alpha chain (EC 3.6.3.14) (Variovorax sp. SCN45)
MQLNPAEISELIKSRIEGLGVSANIRNEGTVVSVTDGIVRVHGLSDAMQGEMLEFPPSAD
GTPSFGLALNLERDSVGAVILGEYEHISEGDTVKCTGRILEVPVGPELIGRVVNALGQPI
DGKGPINAKMTDVIEKVAPGVIARKSVDQPMQTGLKSVDSMVPVGRGQRELIIGDRQTGK
TAVAIDAIINQKGQNMTCVYVAIGQKASSIKNVVRALEQAGAMDYTIVVAASASESAAMQ
YVSAYSGCTMGEYFRDRGQDALIVYDDLSKQAVAYRQVSLLLRRPPGREAYPGDVFYLHS
RLLERAARVNADYVEAFTKGEVKGKTGSLTALPIIETQAGDVSAFVPTNVISITDGQIFL
ETNLFNAGIRPAINAGISVSRVGSSAQTNLVKNQSGGIRTDLAQYRELAAFAQFASDLDE
ATRKQLDRGARVTELLKQTQYNPLPISLMSATLFAVNKGFMDDIDVKKVLSFEHGFHQYL
KSSHASLLETMEKHGAKWDVKPAKADDSDAKKALKKACQDAEAELLAAATSFKKSFA