Protein Info for GFF3680 in Variovorax sp. SCN45

Annotation: ATP synthase F0 sector subunit a (EC 3.6.3.14)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 289 transmembrane" amino acids 42 to 61 (20 residues), see Phobius details amino acids 99 to 117 (19 residues), see Phobius details amino acids 153 to 172 (20 residues), see Phobius details amino acids 227 to 254 (28 residues), see Phobius details amino acids 260 to 282 (23 residues), see Phobius details TIGR01131: ATP synthase F0, A subunit" amino acids 36 to 284 (249 residues), 146.4 bits, see alignment E=6.3e-47 PF00119: ATP-synt_A" amino acids 44 to 282 (239 residues), 197.1 bits, see alignment E=1.8e-62

Best Hits

Swiss-Prot: 71% identical to ATP6_AZOSB: ATP synthase subunit a (atpB) from Azoarcus sp. (strain BH72)

KEGG orthology group: K02108, F-type H+-transporting ATPase subunit a [EC: 3.6.3.14] (inferred from 93% identity to vap:Vapar_4876)

MetaCyc: 48% identical to ATP synthase Fo complex subunit a (Escherichia coli K-12 substr. MG1655)
ATPSYN-RXN [EC: 7.1.2.2]; RXN0-7041 [EC: 7.1.2.2]

Predicted SEED Role

"ATP synthase F0 sector subunit a"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.14

Use Curated BLAST to search for 3.6.3.14 or 7.1.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (289 amino acids)

>GFF3680 ATP synthase F0 sector subunit a (EC 3.6.3.14) (Variovorax sp. SCN45)
MAAENAAEHSQTAGEYIVHHLTHLQSSKPGGVADFTVFNFDSLFFSIALGILGCWLLWLA
ARKATSGVPGRFQAAVELLVEMVDQQAKGIVHNATSRKFVAPLALTIFVWIFLLNAMDLF
PVDLFPAIWAKIFGLAGEDPHHAYLRVVPTADLSVTMGMSVAVLLICIYYNIKIKGAGGW
IHELFTAPFGNHWALYPFNFAMQMIEFIAKTVSHGMRLFGNMYAGELIFLLIALMGGAWS
MSATGIGLAIGHIIAGTAWAIFHILIITLQAFVFMMLTLVYIGQAHDHH