Protein Info for GFF3677 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 709 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 12 to 693 (682 residues), 1054.6 bits, see alignment E=0 PF01138: RNase_PH" amino acids 17 to 146 (130 residues), 103.7 bits, see alignment E=2.6e-33 amino acids 326 to 458 (133 residues), 99.8 bits, see alignment E=4.3e-32 PF03725: RNase_PH_C" amino acids 149 to 211 (63 residues), 45 bits, see alignment E=2.1e-15 amino acids 463 to 531 (69 residues), 25.9 bits, see alignment E=1.9e-09 PF03726: PNPase" amino acids 244 to 322 (79 residues), 65.3 bits, see alignment E=1.4e-21 PF00013: KH_1" amino acids 559 to 616 (58 residues), 47.4 bits, see alignment 3.3e-16 PF00575: S1" amino acids 621 to 692 (72 residues), 59.2 bits, see alignment E=1.1e-19

Best Hits

Swiss-Prot: 86% identical to PNP_ACIAC: Polyribonucleotide nucleotidyltransferase (pnp) from Acidovorax citrulli (strain AAC00-1)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 86% identity to aav:Aave_1259)

MetaCyc: 65% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (709 amino acids)

>GFF3677 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSMFNKVTKSFQWGPHQVTMETGEIARQSTGAVLVNIDDTVVLATVVAKTEAKAGQDFFP
LTVDYTEKSYAAGKIPGSFFKREGRPSELETLTCRLIDRPIRPLFPEGFYNEVQVIVHVV
SLNPEVQADIAAMIATSAALSVSGIPFNGPIGAARVGYVNGEYVLNPGQTQLKDSQLDLV
VAGTEAAVLMVESEADQLSEEVMLGAVVYGHDMGKIAIAAINDLVREAGKPAWDWQPPAK
DEPLIAKIDALAKAKLEAAYQIRNKQARTQACRTAYADVFAALKAEGVDFDSVAVEALLF
EIEAKIVRGQILAGEPRIDGRDTRTVRAIEIRNGVLPRTHGSALFTRGETQALVVATLGT
DRDAQRIDALAGEFEDRFMLHYNMPPFATGEAGRFGTPKRREIGHGRLAKRALIAALPSK
EDFPYTMRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLIKDGNRFAVL
TDILGDEDHLGDMDFKVAGTTNGISALQMDIKIQGITKEIMQVALAQAKEARMHILGKMQ
DAMGEAKTEVSNFAPKLFTMKINPEKIRDVIGKGGSVIRALTEETGTQINIDEDGTITIA
STDSAKADEAKRRIEQITAEVEVGKVYEGPVTKILDFGALINLLPGKDGLLHISQIAHER
VEKVTDYLSEGQVVRVKVLETDEKGRVKLSMKALAERNTGEQAPQQPQS