Protein Info for GFF3670 in Xanthobacter sp. DMC5

Annotation: Chaperone protein ClpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 881 TIGR03346: ATP-dependent chaperone protein ClpB" amino acids 6 to 870 (865 residues), 1389.3 bits, see alignment E=0 PF02861: Clp_N" amino acids 17 to 68 (52 residues), 49.3 bits, see alignment 2.4e-16 amino acids 94 to 145 (52 residues), 51.3 bits, see alignment 5.6e-17 PF00004: AAA" amino acids 204 to 317 (114 residues), 44.2 bits, see alignment E=1.5e-14 amino acids 604 to 722 (119 residues), 33.3 bits, see alignment E=3.4e-11 PF17871: AAA_lid_9" amino acids 343 to 444 (102 residues), 121.5 bits, see alignment E=7.4e-39 PF07724: AAA_2" amino acids 598 to 775 (178 residues), 235.1 bits, see alignment E=2.6e-73 PF00158: Sigma54_activat" amino acids 601 to 720 (120 residues), 21.6 bits, see alignment E=8.2e-08 PF07728: AAA_5" amino acids 603 to 722 (120 residues), 50.7 bits, see alignment E=1.1e-16 PF10431: ClpB_D2-small" amino acids 781 to 861 (81 residues), 101.1 bits, see alignment E=1.6e-32

Best Hits

Swiss-Prot: 80% identical to CLPB_RHOPA: Chaperone protein ClpB (clpB) from Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)

KEGG orthology group: K03695, ATP-dependent Clp protease ATP-binding subunit ClpB (inferred from 88% identity to azc:AZC_4200)

Predicted SEED Role

"ClpB protein" in subsystem Protein chaperones or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (881 amino acids)

>GFF3670 Chaperone protein ClpB (Xanthobacter sp. DMC5)
MNFEIYTERARGFVQSAQSLAVREGNQQFTPEHLLKVLMDDPEGLCSGLIQRAGGNPVLV
RAEVEAALKKLPKVSGGSGQVYLSQQLAKVFETAEQAAKKAGDGYVTVERLLLALAIEKD
TDAGKMLAKAGVTPQTLNAAIEALRKGRTADSATAENAYDALKKYARDLTQAVRDGKLDP
VIGRDEEIRRTIQVLARRTKNNPVLIGEPGVGKTAIVEGLARRIVDGDVPESLKGKSLLA
LDMGSLIAGAKYRGEFEERLKSVLSEVEGAEGGIILFIDEMHTLVGAGKTDGAMDASNLL
KPALARGELHCVGATTLDEYRKHVEKDAALARRFQPVFVSEPTVEDTVSILRGLKEKYEL
HHGVRITDSALVSAAVLSNRYITDRFLPDKAIDLVDEAASRLRMQVDSKPEELDSLDREI
MRRKIEQEALKKETDTASKDRLKRSEKELADLEEKASVLTSKWKSEKEKLGEATTVKAKL
DQARADLATAQRQGEYQKAGELTYSVIPELEKKLADIEKAGGTGGGSMVEEAVTPSHIAG
VVSRWTGVPVDKMLEGEREKLLRMELELAKRVVGQSEAVAAVATAVRRARAGLQDPNRPI
GSFLFLGPTGVGKTELTKALAEFLFDDETAMVRIDMSEYMEKHSVSRLIGAPPGYVGYDE
GGALTEAVRRRPYQVVLFDEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNVLIIMTS
NLGAEFLADPRQPMGFKVPGHEGEQVVSDEEAYDMVMGAVRRHFRPEFINRIDEIVMFHR
LKRDQMDHIVDIQLGRLRKLLEDRKITIELSPEARSFLAEKGYDPAYGARPLKRTIQKLV
QDPLAEKILGGEILDGAKVKVGLDGGHLTFASEAAKVDQAA