Protein Info for GFF3669 in Sphingobium sp. HT1-2

Annotation: D-arabinose-5-phosphate isomerase (EC 5.3.1.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 335 PF01380: SIS" amino acids 57 to 185 (129 residues), 93.5 bits, see alignment E=9.8e-31 TIGR00393: sugar isomerase, KpsF/GutQ family" amino acids 59 to 325 (267 residues), 308.9 bits, see alignment E=1.7e-96 PF00571: CBS" amino acids 214 to 270 (57 residues), 26.4 bits, see alignment E=7.3e-10 amino acids 280 to 332 (53 residues), 34.7 bits, see alignment 1.9e-12

Best Hits

Swiss-Prot: 47% identical to KDSD_PSEAE: Arabinose 5-phosphate isomerase KdsD (kdsD) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K06041, arabinose-5-phosphate isomerase [EC: 5.3.1.13] (inferred from 81% identity to sch:Sphch_2558)

MetaCyc: 44% identical to D-arabinose 5-phosphate isomerase GutQ (Escherichia coli K-12 substr. MG1655)
Arabinose-5-phosphate isomerase. [EC: 5.3.1.13]

Predicted SEED Role

"Arabinose 5-phosphate isomerase (EC 5.3.1.13)" in subsystem KDO2-Lipid A biosynthesis (EC 5.3.1.13)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.3.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (335 amino acids)

>GFF3669 D-arabinose-5-phosphate isomerase (EC 5.3.1.13) (Sphingobium sp. HT1-2)
VSTVSKIVPFGSGAHIVETACSSLALAARGLNTLEAKFSDREFSAAFLRLVGIIINVRGR
VIVTGIGKSGIVARKMTATLTSTGTPALFLHPADAGHGDLGMVTPDDVVLMLSHSGESTE
LGPIIHYCKRFGVPLLAMTAQAQSTVAEAADVCMLMPAVREACPNMLAPTTSTTLQMAFG
DALAMSLMEMRGFSADDFYKFHPNGRLGAQLVKVRELMSAGSDVPRVCEDSSLLDATIEM
TRARLGGTAIVDQGGQLIGAFTDGDLRRAVTGKQNLTDAVGRFMTVTPQSIGPDELASEA
LRIMHDRNITLLFVCEDGDLVGALHMHDLLHAGVA