Protein Info for PS417_18550 in Pseudomonas simiae WCS417

Annotation: folylpolyglutamate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 25 to 421 (397 residues), 306.3 bits, E=2.2e-95
• PF08245: Mur_ligase_M
  • amino acids 47 to 188 (142 residues), 44.1 bits, E=1.2e-15

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 96% identity to pfs:PFLU4186)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• superpathway of chorismate metabolism (44/59 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (8/10 steps found)
• folate polyglutamylation (4/5 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Sequence Analysis Tools

See A0A1N7U9A1 at UniProt or InterPro

Protein Sequence (435 amino acids)

>PS417_18550 folylpolyglutamate synthase (Pseudomonas simiae WCS417)
MTQRTLGEWLAYLEQLHPSAIDMGLERSQQVAARLGLGRPAPRVITVTGTNGKGSTCAFV
AALLQAQGLKVGVYNSPHLLRYNERVQLNGVEATDEQLCEAFAALDAGRGEISLTYFEMG
TLAAFWLFERAQLDVVVLEVGLGGRLDTVNVVDADLALVTSIGVDHADYLGNTRESVAYE
KAGIFRQGKPALCGDLDPPHTLLDKVRELDCPFFLRGRDFNLEIGAQHWQWRGRDARGQV
VELCDLPLLNLPMENAALALQAYLLLDLPWNAEQIAATLLSTRVVGRLDRREFQWQGKRL
NLLLDVGHNPHAAEYLAQRLSRTPPVGRRLAVFGLLADKDLDGVVAPLLGSVQAWAVAPL
DTSRSRPAAELEVALQNLGAPVASYASVTDALEAQCAVATPDDEILLFGSFYCVAEALEW
LARRSTEEAAHGITG