Protein Info for GFF3614 in Xanthobacter sp. DMC5

Annotation: ATP-dependent DNA helicase Rep

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 479 PF00580: UvrD-helicase" amino acids 81 to 148 (68 residues), 24.5 bits, see alignment E=4.3e-09 PF01443: Viral_helicase1" amino acids 417 to 466 (50 residues), 21.1 bits, see alignment 5e-08 PF13538: UvrD_C_2" amino acids 421 to 467 (47 residues), 52.3 bits, see alignment 8.2e-18 PF13361: UvrD_C" amino acids 421 to 470 (50 residues), 37.2 bits, see alignment 5e-13

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 94% identity to bra:BRADO1248)

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (479 amino acids)

>GFF3614 ATP-dependent DNA helicase Rep (Xanthobacter sp. DMC5)
VVEAPGGCGKTFQGAAFAADVSSTLGAGRLLILTHTHAACDVFGDRTKGLSGLEIRTIDS
LIGQIAEAYPEPGLIAGALPDYDRNSRWAAGLLKRRPFIADMLARRYPMVVCDEHQDASA
EQHAVIESLHGAGARVRAFFDPMQRIYGAGATEAQGQADDSRLAAFRAAADVAESLDVPH
RWTGGGEELGGWILESRASLAAGGKLRLTGKLPRGLKVVFADNTAQRNLGFQLDAQDRRP
LDPAFKGAQPLLILSHHNDTVQAIRAFLGRSMPIWEGHTRSALPALVQSLAACHDAAAVA
KAAVGFVQGVCTGFSDSQFATRFQAEVASGCAKPARGKPALIQSLARRIIERPNHAGVGA
FLQDLQHATKFQTEFASVRIDYPREFWEAVRLGAATDPRAGLAEQSRHNALTRRLPPLRA
VSTIHKAKGLEAPNVVIMPCDGTTFREKDRRLLYVAISRASRNLTLVVSRSNPSPIIEL