Protein Info for GFF3604 in Sphingobium sp. HT1-2

Annotation: ATP-dependent DNA helicase RecG (EC 3.6.4.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 PF04851: ResIII" amino acids 275 to 430 (156 residues), 38.8 bits, see alignment E=1.8e-13 PF00270: DEAD" amino acids 275 to 435 (161 residues), 83.1 bits, see alignment E=3.8e-27 PF00271: Helicase_C" amino acids 475 to 585 (111 residues), 61.9 bits, see alignment E=1.4e-20 PF19833: RecG_dom3_C" amino acids 614 to 675 (62 residues), 47.3 bits, see alignment 3.4e-16

Best Hits

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 86% identity to sch:Sphch_0523)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (700 amino acids)

>GFF3604 ATP-dependent DNA helicase RecG (EC 3.6.4.12) (Sphingobium sp. HT1-2)
MTVPAALEKPQIAMRPDILNPLFAEISALKGIGPALARPLERLGLARAVDVAFHLPVNYV
DRKLIDELDMADAGKVIGIMLTPVDYRASGNARAPFRVQAVDAHGNAVSLVYFGRNSAWP
RKLLPLNEPKFVSGKLEAYGDNLQMVHPDYVLPPEEADTVPARESVYGLSEGLTNNRMRD
LVGQALARAPELPEWIEPSLLASKGWPAWRAALERFHADPTDAQARERLAYDEIFAGQLA
LMLVRQSSRRRRGVPISGDGRLRAMLKLPFAPTGAQRRAIGEIEGDMAQATPMLRLLQGD
VGSGKTLVALMALLNAVEAGMQGAMLAPTEILARQHYETLRKMASGLPIEIAILTGREKG
KVREATLMGLANGSIHILVGTHAIFQEKVQYKALGLAVIDEQHRFGVAQRMMLASKAERS
PHLLVMTATPIPRTLTLTYYGEMDVSRLDEMPPGRQPIQTLVMSANRLDEVVEGLARHVE
GGGQAYWVCPLVEESETSDQAAAEMRAETLRQRFGDRVGLVHGKMKGPDKDAAMEAFAAN
RTQILVATTVIEVGVDVPNSSLIVIEGAERFGLAQLHQLRGRVGRGDKPSVCLLLRGNAL
GETSRARLALMRETNDGFRIAEEDLKLRGAGEVLGTRQSGEAELKIATPEHVSALVDAAR
DDAHLLIDRDGGLDSERGQAARTCLYLFEKDAAVGLLRGG