Protein Info for GFF3590 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Urocanate hydratase (EC 4.2.1.49)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 TIGR01228: urocanate hydratase" amino acids 13 to 563 (551 residues), 936.8 bits, see alignment E=1.9e-286 PF17391: Urocanase_N" amino acids 15 to 141 (127 residues), 199.8 bits, see alignment E=1.7e-63 PF01175: Urocanase" amino acids 144 to 358 (215 residues), 270.8 bits, see alignment E=1.2e-84 PF17392: Urocanase_C" amino acids 361 to 555 (195 residues), 309.2 bits, see alignment E=1.5e-96

Best Hits

Swiss-Prot: 76% identical to HUTU_TRIRP: Urocanate hydratase from Trifolium repens

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 89% identity to pol:Bpro_1042)

MetaCyc: 75% identical to urocanase subunit (Pseudomonas putida)
Urocanate hydratase. [EC: 4.2.1.49]

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (565 amino acids)

>GFF3590 Urocanate hydratase (EC 4.2.1.49) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VTTAIDPRLDPTRSIRAPRGSTLNCKNWIIEAAYRMIQNNLDPEVAENPQHLVVYGGIGR
AARDWACFDQILASLKNLDEDETLLVQSGKPVGVFKTHVNVPRVLLANSNLVPKWANWEH
FNELDRQGLFMYGQMTAGSWIYIGSQGIVQGTFETFVEAGRQHYGNDLAGRWFLTAGLGG
MGGAQPLAATLAGACSLNIECQQSSIDFRLRTRYVDKQAADIDDALALIRHHTARKEAVS
IALLGNAAEVLPELVKRAKAGGLRPDLVTDQTSAHDLINGYLPAGWSVAQWKAAQHDSTQ
HARLVDAAAKSCAVHVQAMLDFHAMGIPTVDYGNNIRQVAFDQGVGNAFDFPGFVPAYIR
PLFCEGKGPFRWVALSGDPQDIHTTDAKIKELFPHNHHTHRWLDMARERIAFQGLPARIC
WLGLGERHLAGLAFNEMVRTGELKAPIVIGRDHLDTGSVASPNRETEGMRDGTDAVSDWP
LLNALLNTAGGASWVSLHHGGGVGMGYSQHAGMVIVADGSDEAAERLARVLVNDCGSGVM
RHADAGYELAIATAKKHGLKLPMVA