Protein Info for GFF357 in Xanthobacter sp. DMC5

Annotation: putative xanthine dehydrogenase subunit D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02965: xanthine dehydrogenase, molybdopterin binding subunit
  • amino acids 5 to 756 (752 residues), 1098 bits, E=0
• PF01315: Ald_Xan_dh_C
  • amino acids 19 to 125 (107 residues), 95 bits, E=5.5e-31
• PF02738: MoCoBD_1
  • amino acids 145 to 376 (232 residues), 276.9 bits, E=1.8e-86
• PF20256: MoCoBD_2
  • amino acids 406 to 688 (283 residues), 329.3 bits, E=3.5e-102

Best Hits

KEGG orthology group: K13482, xanthine dehydrogenase large subunit [EC: 1.17.1.4] (inferred from 84% identity to xau:Xaut_3760)

Predicted SEED Role

"Xanthine dehydrogenase, molybdenum binding subunit (EC 1.17.1.4)" in subsystem Purine Utilization (EC 1.17.1.4)

MetaCyc Pathways

• adenosine nucleotides degradation II (4/5 steps found)
• guanosine nucleotides degradation II (3/4 steps found)
• guanosine nucleotides degradation III (3/4 steps found)
• inosine 5'-phosphate degradation (3/4 steps found)
• purine nucleotides degradation II (aerobic) (8/11 steps found)
• adenosine nucleotides degradation I (5/8 steps found)
• guanosine nucleotides degradation I (2/4 steps found)
• ureide biosynthesis (4/7 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• superpathway of guanosine nucleotides degradation (plants) (3/6 steps found)
• purine nucleotides degradation I (plants) (7/12 steps found)
• superpathway of purines degradation in plants (9/18 steps found)
• caffeine degradation III (bacteria, via demethylation) (1/7 steps found)
• theophylline degradation (1/9 steps found)
• caffeine degradation IV (bacteria, via demethylation and oxidation) (1/10 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.1.4

Use Curated BLAST to search for 1.17.1.4

Protein Sequence (764 amino acids)

>GFF357 putative xanthine dehydrogenase subunit D (Xanthobacter sp. DMC5)
MTRAVHVPARHESANAHVSGRAVYLDDMPEAPGLLHAALVLSPHARARIVAIDLSAARAL
PGVVAVVAGDIPGVNDIAPIRANEPLLADAEADYVGHPVAAVAAPTLDAARAAAALVRVE
YEALPALLDLDAAYEAGARVAPDQQVGRGDVASALAGAPLRLSGEVRCGGQDHFYLEGQI
AIAIPGEDRDMLVYSSTQHPTEAQHGVAHVLGLPFAAVTVEVRRMGGAFGGKESQATIIA
AIAALLAHHAGKPVKLRLPREVDMEATGKRHPFRVRWEAGFDGDGRIAGLDMEFAADCGN
VADLSPAVVSRALCHADNCYFIPATRFRGVLVKTNTVSNTAFRGFGAPQGMLAIEAVMDE
IARHLGKPIEVVRAANHYGAAPRNITPYGQEVEDSLIEEAMAHLDAKANLAAWRAEIDAF
NAQSPVIRKGLATMPIKFGVSFNLTTLNQAGALVHVYTDGSVHLNHGGTEMGQGLFVKVA
QVVADAFGIPLDHVRVSATSTAKVPNTSPTAASSGSDLNGMAALIAAKEIRGRMAGVVAA
HFDAPVEEVVFAEGRVSVGNRSLSFGEAAHMAWQQRVPLSATGFYKTPKIHFDLAKSEGR
PFYYYTYGAAAAEVAVDTLSGAVRVLRAEIVGDCGRSLNPAIDIGQIEGAFVQGMGWLTC
EELKWDAKGRLATRGPSTYKIPGSRDVPPVLNVHLLDKPNAEETVLRSKAVGEPPLMLAI
SVWLAIRNVIAGLPGDGPVHLDAPATPERVLAAVSARREGQARA