Protein Info for GFF3568 in Xanthobacter sp. DMC5

Annotation: Release factor glutamine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 303 PF06325: PrmA" amino acids 44 to 294 (251 residues), 179.7 bits, see alignment E=3.4e-56 PF13489: Methyltransf_23" amino acids 152 to 286 (135 residues), 40.2 bits, see alignment E=1.1e-13 PF05175: MTS" amino acids 157 to 240 (84 residues), 37.2 bits, see alignment E=8.5e-13 PF01135: PCMT" amino acids 159 to 258 (100 residues), 26.3 bits, see alignment E=2.1e-09 PF13847: Methyltransf_31" amino acids 160 to 259 (100 residues), 30.8 bits, see alignment E=8e-11 PF13649: Methyltransf_25" amino acids 161 to 254 (94 residues), 35.8 bits, see alignment E=3.7e-12 PF08241: Methyltransf_11" amino acids 162 to 257 (96 residues), 21.4 bits, see alignment E=1.1e-07

Best Hits

Swiss-Prot: 53% identical to PRMA_BRADU: Ribosomal protein L11 methyltransferase (prmA) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K02687, ribosomal protein L11 methyltransferase [EC: 2.1.1.-] (inferred from 85% identity to xau:Xaut_1424)

Predicted SEED Role

"Ribosomal protein L11 methyltransferase (EC 2.1.1.-)" in subsystem Heat shock dnaK gene cluster extended or Ribosome biogenesis bacterial (EC 2.1.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (303 amino acids)

>GFF3568 Release factor glutamine methyltransferase (Xanthobacter sp. DMC5)
MLEGLPPNDATHVMRVEAPRRQAQRIADLISETFDPAEVAVANFERTDDDWVVEVYAGAA
FDADTLRELVEIAAGPELAATVTFGEIEQKDWVAASLEGLAPVAVGPVVVHGSHDRGRVG
PNRIGIEIEAALAFGTGHHGTTQGCLHAIVNAARRGRPQRVLDVGTGTGVLAIAAERLFH
TEIVGSDIDIIAVRTAKANAAANKAPGVRMVHAAGAGARAIRIHGPYDLILANILLPPLK
RLARPLASLLAPGGRIVLSGLLTAQVPAALAAYRAQGLHLVSQRHIEGWATLTLAARSDR
PRG