Protein Info for PS417_18240 in Pseudomonas simiae WCS417
Annotation: nitrile hydratase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 93% identical to NHAB_PSECL: Nitrile hydratase subunit beta (nthB) from Pseudomonas chlororaphis
KEGG orthology group: K01721, nitrile hydratase [EC: 4.2.1.84] (inferred from 93% identity to pfs:PFLU3210)MetaCyc: 93% identical to nitrile hydratase beta subunit (Pseudomonas chlororaphis B23)
Nitrile hydratase. [EC: 4.2.1.84]; 4.2.1.84 [EC: 4.2.1.84]; 4.2.1.84 [EC: 4.2.1.84]; 4.2.1.84 [EC: 4.2.1.84]
Predicted SEED Role
"Cobalt-containing nitrile hydratase subunit beta (EC 4.2.1.84)" (EC 4.2.1.84)
MetaCyc Pathways
- aldoxime degradation (3/3 steps found)
- acrylonitrile degradation I (2/2 steps found)
- indole-3-acetate biosynthesis IV (bacteria) (2/2 steps found)
- superpathway of acrylonitrile degradation (2/3 steps found)
- indole-3-acetate biosynthesis II (5/12 steps found)
KEGG Metabolic Maps
- 1,4-Dichlorobenzene degradation
- Benzoate degradation via CoA ligation
- Cyanoamino acid metabolism
- Styrene degradation
- Tryptophan metabolism
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.84
Use Curated BLAST to search for 4.2.1.84
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1N7UNM8 at UniProt or InterPro
Protein Sequence (220 amino acids)
>PS417_18240 nitrile hydratase (Pseudomonas simiae WCS417) MDGFHDLGGFQGFGKVPHTINSLDYKQVFKQDWEHLAYSLMFIGADHLKKFSVDEVRHAV ERLDVRQHVGTQYYERYVIATATLLVETGVITQAELDQALGSPFKLANPAHAEGRPAITG RPHFEVGDRVVVRDEYVAGHTRMPAYVRGKAGVVLHRTSEQWPFPDAIGHGDVSAAHQPT YHVEFRVKDLWGDAADDGLVVVDLFESYLDHADVAQAVKA