Protein Info for GFF3560 in Xanthobacter sp. DMC5

Annotation: NAD-specific glutamate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21075: GDH_ACT1
  • amino acids 37 to 175 (139 residues), 105.5 bits, E=8.6e-34
• PF21073: GDH_HM1
  • amino acids 341 to 400 (60 residues), 72.6 bits, see alignment (E = 7.7e-24)
• PF21076: GDH_ACT2
  • amino acids 404 to 493 (90 residues), 67.2 bits, 6.2e-22
• PF21079: GDH_HM2
  • amino acids 511 to 546 (36 residues), 36.8 bits, see alignment (E = 1.3e-12)
• PF21077: GDH_ACT3
  • amino acids 552 to 627 (76 residues), 65.8 bits, 9.3e-22
• PF21078: GDH_HM3
  • amino acids 646 to 711 (66 residues), 92.1 bits, see alignment (E = 7.4e-30)
• PF05088: Bac_GDH_CD
  • amino acids 731 to 1225 (495 residues), 845.6 bits, E=4.6e-258
• PF21074: GDH_C
  • amino acids 1270 to 1602 (333 residues), 327.1 bits, E=5.6e-101

Best Hits

KEGG orthology group: K00260, glutamate dehydrogenase [EC: 1.4.1.2] (inferred from 70% identity to azc:AZC_4508)

Predicted SEED Role

"NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.2)

MetaCyc Pathways

• methylaspartate cycle (14/19 steps found)
• L-glutamate degradation I (1/1 steps found)
• L-alanine degradation II (to D-lactate) (2/3 steps found)
• ethene biosynthesis IV (engineered) (1/3 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (6/10 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (3/7 steps found)
• 4-aminobutanoate degradation V (2/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.2

Protein Sequence (1608 amino acids)

>GFF3560 NAD-specific glutamate dehydrogenase (Xanthobacter sp. DMC5)
MNVMGFQAGAHGLHDETALAAVKALIERDAPHLPAGFADTFLAAVAPEDLAPLPAACIAL
LLREAWAHVAARPKGQPSVRVFNPPLPGPAITVIEAVNDDMAFLFDSVAGELADRGLVVK
LAAHPILHVERDGDGGVRGLESSGPGSLSEENRESLIHIHVAAIDEAAAAELKAGLDRTL
ADVRAANLDFSAMRKQVKSLSKGYRREKRPYGDDAREEAADVLEWLTEDNFIFLGLRHYE
LSEDGLKAEANSGLGILRNPDVHELRLGDAPVVTTPEIRAFLEGPTPLLFTKASLRSRVH
RRDVMDYVGIKAHDEKGRLIGEVRLIGLFASTAYTHSLREIPYLRLKADSVIRGAGLEPD
SHSARALSTVLETYPRDDLFQIDVPTLLVHAREILSLYDRPRLKVLARADRFDRFVSVLT
FLPRERFDAQVRDRVGAILEKSYGGTVTSLEQAFVAGLPLTRVHYDIGRTEGRIPEVDKG
ELERAVADAILTWRDRLSAAVANVPGIDAGDAARLSRRYVEAFDPGYRAAYGVPTALADI
ELLERLSEERPVALDFYQREGDDDRRIALRLLSYGAPLPLAARVPMLENMGLKAINERTF
RIEPAGGPKSWVHDMSLERADGGSISVEGSAARLEDCLNAVLRGAAENDGYNALVLDAGL
SFREAALVRALGRYLRQAGIPFSQDYIWQTLNRHGALAHAIVGLFQARFDPALDTSAEAR
AAREAPLRAAIDDALAAVSSLDEDRILRRFVNLVDATLRTTYYQKDAQGRLREPIAIKFE
SAKVEGLPLPRPLYEVFVYSPRVEGVHLRFGKVARGGLRWSDRPQDFRTEILGLVKAQQV
KNAVIVPVGAKGGFVPKRLPAGGSREAVFAEGTAAYEVFVSSLLDLTDNLKGGAVVPPAD
TVRLDGDDPYLVVAADKGTATFSDTANAISARHGFWLDDAFASGGSVGYDHKAMGITARG
AWEAVKRHFREMDIDIQTTPVTVAGVGDMSGDVFGNGMLLSSALKLVAAFDHRHIFLDPE
PDPARAFAERRRLFDLPRSSWADYDASLISKGGGIFPRSAKSIDLSEEVRALLRFPKASA
TPAELINAILKAPVDLLFFGGIGTYVRASAETDAQAGDRANDAVRIAAADLNARVIGEGA
NLGMTQRGRIEAARKGVRLNTDAIDNSAGVNTSDVEVNIKIALAKPLEMGDLSVPDRAAL
LKQMTDEVARLVLVNNYDQTLALSLAELKGADDLAFQQRLMQMLEARGELDRSVEFLPSD
AEIADRRSRGEHLTRPELAVLLAYAKLALKDDLIGSSVPDDAYLSGELVHYFPHELQDRY
PHAIETHRLHREIIATGLANAIVNQGGPSAVARISDQTGGDAASIARAFAAVRDSYHLPE
LTAAIDALDNKVDGHVQLQLYAAVQDLMVGRTIWFLGNVSFADGIAGVVERYRSGVAAVA
AALDESLPEVWRKDRAARVADLVAQRVPEELARRVASMPALAAASDIALIAETTGKAIPS
AASTFFAAGRYFAIDDLLTQSRSIMAPDYYDRLALDRSLAQLETFLRRITEAMLAEGVSG
EAAVEAYVARRREEVERMRRTVRDIAQSGLSLSKLTLAASLLGDLVKA