Protein Info for PS417_18230 in Pseudomonas simiae WCS417
Annotation: amidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 84% identical to AMID_PSECL: Amidase from Pseudomonas chlororaphis
KEGG orthology group: K01426, amidase [EC: 3.5.1.4] (inferred from 94% identity to pfs:PFLU3208)MetaCyc: 84% identical to nicotinamidase (Pseudomonas chlororaphis B23)
Nicotinamidase. [EC: 3.5.1.19]
Predicted SEED Role
"Amidase clustered with urea ABC transporter and nitrile hydratase functions" in subsystem Amidase clustered with urea and nitrile hydratase functions
MetaCyc Pathways
- aldoxime degradation (3/3 steps found)
- acrylonitrile degradation I (2/2 steps found)
- indole-3-acetate biosynthesis III (bacteria) (2/2 steps found)
- indole-3-acetate biosynthesis IV (bacteria) (2/2 steps found)
- NAD salvage pathway I (PNC VI cycle) (5/7 steps found)
- L-arginine degradation X (arginine monooxygenase pathway) (2/3 steps found)
- superpathway of acrylonitrile degradation (2/3 steps found)
- NAD salvage pathway V (PNC V cycle) (3/5 steps found)
- superpathway of NAD biosynthesis in eukaryotes (9/14 steps found)
- NAD salvage (plants) (5/11 steps found)
- indole-3-acetate biosynthesis II (5/12 steps found)
KEGG Metabolic Maps
- Arginine and proline metabolism
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Cyanoamino acid metabolism
- Nicotinate and nicotinamide metabolism
- Phenylalanine metabolism
- Styrene degradation
- Tryptophan metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.19, 3.5.1.4
Use Curated BLAST to search for 3.5.1.19 or 3.5.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A1N7U3T3 at UniProt or InterPro
Protein Sequence (504 amino acids)
>PS417_18230 amidase (Pseudomonas simiae WCS417) MAIVRPTLEQLLDIASRLHMQLSPEHAREYLPLMQASFDAYDLIDELPDFIPPVRYERSS GYRPSAAHNPLNAWYVRTEVSGARDGKLAGKTVALKDNISLAGVPMMNGAKPLEGHVPSF DATVVTRLLDAGATILGKATCEHYCLSGGSHTSDPAPVHNPYRHGFASGGSSSGSAALVA AGEVDLAVGGDQGGSIRIPSAFCGTYGMKPTHGLVPYTGIMAIEATIDHAGPITANVRDN ALMLEVMAGADGLDPRQAAPQVDTYSDYLARGVSGLKIGVLREGFELANQDPRVASSVRN AIARFSQLGAQVEEVSVPEHAIAGALWHPIGCEGLTMQMMHGNGAGFNWKGLYDVGLLDK QAGWRDQADDLSASLKLCMFVGQYGVERYNGRFYAKAQNLRRLARSAYDAALARYDLLVM PTVPIIAQPHPEPDCSITEYVARALEMIGNASAQDITGHPAMSIPCGLVDGLPVGLMLVG KHYAEGTIYQAAAAFEAAVDWKNC