Protein Info for GFF3543 in Xanthobacter sp. DMC5

Annotation: Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 508 transmembrane" amino acids 435 to 454 (20 residues), see Phobius details TIGR03706: exopolyphosphatase" amino acids 14 to 304 (291 residues), 308.7 bits, see alignment E=2.1e-96 PF02541: Ppx-GppA" amino acids 28 to 306 (279 residues), 144.8 bits, see alignment E=3.9e-46 PF21697: Ppx_C" amino acids 314 to 496 (183 residues), 215 bits, see alignment E=9.6e-68

Best Hits

KEGG orthology group: K01524, exopolyphosphatase / guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase [EC: 3.6.1.11 3.6.1.40] (inferred from 90% identity to xau:Xaut_3126)

Predicted SEED Role

"Exopolyphosphatase (EC 3.6.1.11)" in subsystem Phosphate metabolism (EC 3.6.1.11)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.11, 3.6.1.40

Use Curated BLAST to search for 3.6.1.11 or 3.6.1.40

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (508 amino acids)

>GFF3543 Guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase (Xanthobacter sp. DMC5)
LTHAPGRLSHGAPVAVIDIGSNSVRLVVYEGLNRALTPVFNEKALCGLGREVLSTGRLPQ
DAVDRAFAALKRFRALCDAMQVGEVHALATAAARDAQNGPAFIAECEAICGTRIELLTGK
REAELAACGIVAGVHAPDGIVGDLGGGSLELVDVKAGYVGTGVTLPLGGLALQDRSSRST
RKAEKIVRDTIPEAQVLEGLAGRTFYAVGGTWRALARLHMFTKGYPLHVMHGYVIPAREA
LDFTRIVHRADTEALAHIDVISDARRPLLAYGALVLENIVRQGRPSEVVISALGVREGLL
YSLLPEAERAGDPLLIAAAEMNVLRSRSPLHGEELVAWTDGFFASAPVLEEAEEERRLRR
AACLLADVGWRAHPDYRGEQSLNMIAHAAFAGIDHPGRAYVALSVYFRHVGLGADESDTR
ILELVTTGMLDRARILGAAMRVAYVLSAAMPGILPRMPLKVRGDQLALSVPKQLVPLINE
RVQNRLRQLARLIGREPALEQYEEGTAD