Protein Info for GFF3532 in Sphingobium sp. HT1-2

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 151 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF00190: Cupin_1" amino acids 63 to 137 (75 residues), 24.4 bits, see alignment E=2.9e-09 PF07883: Cupin_2" amino acids 69 to 133 (65 residues), 66.1 bits, see alignment E=2.7e-22 PF02311: AraC_binding" amino acids 78 to 127 (50 residues), 24 bits, see alignment E=4.2e-09

Best Hits

Predicted SEED Role

"Mannose-6-phosphate isomerase (EC 5.3.1.8)" in subsystem Alginate metabolism or Mannose Metabolism (EC 5.3.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.8

Use Curated BLAST to search for 5.3.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (151 amino acids)

>GFF3532 hypothetical protein (Sphingobium sp. HT1-2)
MGASGMAGWDGRIVGAGMTILLLPLLLAAAPDAPITPIDKANAEHYVWGGSNDGWHLVKR
DEMSVIQERIVPGGAEVRHYHQRARQFFYVLSGELTLEAGGVTHVLKAGQGLEVPPGVPH
RAQNRSGASVDFLVNSTPKSHGDRVDAPAGK