Protein Info for PS417_18085 in Pseudomonas simiae WCS417

Annotation: general secretion pathway protein GspE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 563 PF05157: MshEN" amino acids 69 to 155 (87 residues), 40.7 bits, see alignment E=2e-14 TIGR02533: type II secretion system protein E" amino acids 88 to 558 (471 residues), 635.7 bits, see alignment E=2.5e-195 PF00437: T2SSE" amino acids 181 to 556 (376 residues), 467.6 bits, see alignment E=2.2e-144

Best Hits

Swiss-Prot: 62% identical to GSPE_XANCP: Type II secretion system protein E (xpsE) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K02454, general secretion pathway protein E (inferred from 91% identity to pfs:PFLU4080)

Predicted SEED Role

"General secretion pathway protein E"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UHT8 at UniProt or InterPro

Protein Sequence (563 amino acids)

>PS417_18085 general secretion pathway protein GspE (Pseudomonas simiae WCS417)
MSSAPIDACQMQIPHTEQVCAWLMQHAGLKTVDLERARRLSTDDLLSLLTRLGLVSEVEL
ARAWAALLGAPLLLADAAPPLLDPLPVLTERFMRHYQVVPMGWTQGGLRVLAANPAQLYP
FQALAYACQVPVWLAIGPRNEVDTLIERYYGQGRSAMGTLIENLDEQGGAEEDIEHLKDM
ASEAPVIRLVNLILQRAVEQRASDIHIEPFENQLKVRYRIDGVLHDAEAPPASSSAAVIS
RVKIMARLDIAERRLPQDGRIMLRIQGKELDLRVSTVPTSFGESVVLRLLDRQTVQFDFP
SLGFDGQRLASFLDLLERPHGILLVTGPTGSGKTTTLYTALSRLNTTERKIITVEDPVEY
QLEGINQIQVKPAIGLDFAGALRSIVRQDPDVIMIGEIRDLETCRIAIQSSLTGHLVLST
LHTNSAAASITRLMDMGVESYLIASTVSGILAQRLVRRLDPVTRVAFEAPPELIAEHGLD
RLTEQRPILLYRGDYHGRSALTELLVMNDELRSLLMRHADAATLEQAARRAGLRTLYEEG
LRQALAGITSLEEVLRVTRGEGA