Protein Info for GFF3529 in Xanthobacter sp. DMC5

Annotation: Aspartate--tRNA(Asp/Asn) ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 591 TIGR00459: aspartate--tRNA ligase" amino acids 5 to 584 (580 residues), 577.3 bits, see alignment E=1.6e-177 PF01336: tRNA_anti-codon" amino acids 21 to 104 (84 residues), 55.6 bits, see alignment E=6.4e-19 PF00152: tRNA-synt_2" amino acids 122 to 560 (439 residues), 365.1 bits, see alignment E=3.9e-113 PF02938: GAD" amino acids 322 to 404 (83 residues), 29.5 bits, see alignment E=1.1e-10

Best Hits

Swiss-Prot: 87% identical to SYDND_AZOC5: Aspartate--tRNA(Asp/Asn) ligase (aspS) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 90% identity to sno:Snov_1120)

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23)" (EC 6.1.1.12, EC 6.1.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (591 amino acids)

>GFF3529 Aspartate--tRNA(Asp/Asn) ligase (Xanthobacter sp. DMC5)
MHRYRSHTCGALSTAEVGQIVRLSGWCHRIRDHGGVLFIDLRDHYGLTQVVVDPDSPAFK
GAEKARSEWVIRIDGKVRPRPEGTENPDLTTGAIEVYALELEVLGPAAELPLPVFGDVEY
PEETRLKYRFLDLRREKLHRNIMTRGAIIDAMRRRMKDQGFFEFQTPILTASSPEGARDF
LVPSRLHPGKFYALPQAPQQYKQLIMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVEQEDVFAAMEPVITGVFEEFAGGKPVTKNWPRIPYAEALRNYGTDKPDLRNPLVMQ
NVSDHFRGSGFKVFARMLEVEKNEVWAIPAPGGGSRAFCDRMNSWAQGEGQPGLGYIMWR
EGGEGAGPLANNIGPERTEAIREQLGLKAGDAAFFVAGDPDKFVKFAGLARTKVGEELNL
VDKDRFELAWIVDFPFYEYSEEEKKVDFSHNPFSMPQGGLEALNTQDPLTIKAFQYDIAC
NGYEIASGGIRNHRPEAMVKAFELAGYDEATVVERFGGMYRAFQYGAPPHGGMAAGVDRI
VMLLCGTTNLREISIFPMNQQALDLLMGAPNEASPKQLRELHIRPAPQAKG