Protein Info for GFF349 in Xanthobacter sp. DMC5

Annotation: Putative ring-cleaving dioxygenase MhqO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 PF00903: Glyoxalase" amino acids 5 to 126 (122 residues), 36.6 bits, see alignment E=2.7e-13 amino acids 153 to 269 (117 residues), 27 bits, see alignment E=2.5e-10

Best Hits

KEGG orthology group: None (inferred from 84% identity to xau:Xaut_3750)

Predicted SEED Role

"Glyoxalase family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (310 amino acids)

>GFF349 Putative ring-cleaving dioxygenase MhqO (Xanthobacter sp. DMC5)
MRSIGLHHVTAIAGPARRNLDFYTRTLGLRLVKKTVNFDDPGTYHFYFGDAAGSPGSILT
FFPWEAAGTGRAGVGETQETAFRIPEAAVSYWTERLIGAGLSPDLPEKRFGETVIAFKDP
DGMRIALVGVPGIEAEPAWAGGEVPEANAIRGMHSVTLLLKEAGATADILTDVFGFEEAG
REGTRIRYRAPGAAVGGIVDIREAGGFLSGRQGRGSVHHVAFRASDDAAQAEMVEKLTAR
HGRHVTEQRDRNYFRSVYFREPGGVLFEIATDDPGFAVDEAPEHLGEALKLPAQYEAHRA
EIERVLPQVA